c-myc基因与辐射诱发小鼠胸腺淋巴瘤的关联性分析  

Analysis on relevance between c-myc gene and thymic lymphomas of mice induced by ionizing radiation

作　　者：于雷[1] 刘永哲[1] 孙世龙[1] 方芳[1] 巩宏伟[1] 陈强[1] 鞠桂芝[1] 

机构地区：[1]吉林大学公共卫生学院卫生部放射生物学重点实验室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2009年第6期971-974,共4页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(30670630);吉林大学研究生创新基金资助课题(20091032)

摘　　要：目的:研究辐射诱发小鼠胸腺淋巴瘤c-myc基因表达的变化,为阐明辐射致癌机制提供理论依据。方法:采用X射线(剂量率0.287Gy.min-1,总剂量7Gy)照射BALB/c小鼠,建立小鼠胸腺淋巴瘤模型,6个月后处死小鼠,取辐射诱发小鼠胸腺淋巴瘤和正常胸腺,分别提取总RNA、合成cDNA和提取总蛋白,采用实时定量PCR方法和Western blotting方法分别检测辐射诱发胸腺淋巴瘤和正常胸腺组织c-myc基因mRNA和蛋白表达。结果:经实时定量PCR检测,胸腺淋巴瘤和正常胸腺组织c-myc基因mRNA相对表达量分别为9.16±4.66和1.16±0.54,两组比较差异有显著性(P<0.01);Western blotting检测结果显示,胸腺淋巴瘤c-myc基因蛋白表达较正常胸腺组织明显增高。结论:c-myc基因与辐射诱发小鼠胸腺淋巴瘤有关联,可能是辐射致癌的易感基因。Objective To study the change of c-myc gene expression in thymic lymphomas of mice induced by ionizing radiation and provide theoretical basis for mechanism of radiation carcinogenesis. Methods The thymic lymphomas models of BALB/c mice were made by X-ray （dose rate： 0. 287 Gy·min^-1 ; total dose： 7 Gy） exposuring, 6 months later they were killed, then total RNA and total protein were extracted from thymic lymphomas and normal thymus tissue and cDNA was synthesized, the expressions of c-myc mRNA and protein in thymic lymphomas induced by ionizing radiation and normal thymus were detected by quantitative real-time PCR and Western blotting respectively. Results The expression of c-myc mRNA in thymic lymphomas （9.16±4.66） was higher than that in normal thymus tissue （1.16±0.54） （P〈0.01） . The expression of c-myc protein in thymic lymphomas was higher than that in normal thymus tissue. Conclusion There is relevance between c-myc gene and thymic lymphomas induced by ionizing radiation, c-myc gene might be susceptibility gene of radiation carcinogenesis

关 键 词：胸腺肿瘤 淋巴瘤 基因 myc 实时定量PCR 电离辐射 

分 类 号：Q345[生物学—遗传学] R733[医药卫生—肿瘤]