电离辐射诱导人乳腺癌细胞自噬与凋亡的关系  

Relationship between autophagy and apoptosis of MCF-7 cells induced by ionizing radiation

作　　者：齐亚莉[1,2] 张震宇[3] 王洪艳[1,2] 李金华[1] 龚守良[1] 

机构地区：[1]吉林大学公共卫生学院卫生部放射生物学重点实验室,吉林长春130021 [2]北华大学公共卫生学院流行病学教研室,吉林吉林132021 [3]武警吉林省总队医院外科,吉林长春130052

出　　处：《吉林大学学报（医学版）》2009年第6期1007-1010,共4页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(39870312)

摘　　要：目的:探讨电离辐射联合自噬和凋亡抑制剂及诱导剂对人乳腺癌细胞株增殖的抑制作用。方法:采用四甲基偶氮唑蓝(MTT)和流式细胞术(FCM)检测经不同剂量(0、2、4、8和10Gy)照射和4Gy、4Gy+自噬抑制剂三甲基腺嘌呤(3-MA)、4Gy+自噬诱导剂(rapamycin)、4Gy+凋亡抑制剂泛半胱氨酸蛋白酶抑制剂(z-VAD-fmk)不同方法处理的人乳腺癌细胞存活和增殖情况,并分析其量效、时效关系。结果:随着照射剂量的增加(4、8和10Gy)及时间的延长(48和72h),乳腺癌细胞的增殖抑制率升高,组间比较差异有显著性(P<0.05或P<0.01)。与4Gy+rapamycin处理组比较,4Gy+3-MA和4Gy+z-VAD-fmk处理组癌细胞增殖抑制率比较差异有显著性(P<0.05或P<0.01),不同方法处理后24、48和72h其抑制率组间比较差异有显著性(P<0.05或P<0.01)。结论:电离辐射联合自噬诱导剂能够促进癌细胞凋亡,而电离辐射联合自噬抑制剂或凋亡抑制剂能够抑制其凋亡。电离辐射可诱导人乳腺癌细胞自噬并促进其凋亡。Objective To detect the inhibitory effects of ionizing radiation combined with autophagy and apoptosis inhibitors and inducers on the proliferation of human breast cancer cell line.Methods MTT and flow cytometry （FCM） were used to detect the surviving and proliferation of MCF-7 cells,which were under 0,2,4,8 and 10 Gy X-ray radiation and different dealing methods 4 Gy,4 Gy ＋ 3-MA,4 Gy ＋ rapamycin,4 Gy ＋ z-VAD-fmk,and the relationship of dose-effects and time-effects was analyzed.Results With the increase of irradiation doses （4,8 and 10 Gy） and the elongation of irradiation time （48 and 72 h）,the inhibitory rates of the proliferation of breast cancer cells were increased ,there were significant differences between various groups（P〈0.05 or P〈0.01）.The inhibitory rates of the proliferation of breast cancer cells in 4 Gy＋3-MA or 4 Gy＋ z-VAD-fmk groups were significantly different from those in 4Gy＋rapamycin group（P〈0.05 or P〈0.01）,and there were significant differences after treated for 24,48 and 72 h between various groups （P〈0.05 or P〈0.01）.Conclusion Ionizing radiation in combination with autophagy inducer could induced the autophagy in human breast cancer cells and promote the apoptosis;the ionizing radiation in combination with autophagy inhibitor or apoptosis inhibitor could inhibit the apoptosis.Thus,ionizing radiation can induce the autophagy in human breast cancer cells,and promote the apoptosis.

关 键 词：电离辐射 自噬 细胞凋亡 乳腺肿瘤 

分 类 号：Q345[生物学—遗传学] R737.9[医药卫生—肿瘤]