聚肌胞对小鼠前列腺癌组织内血管生成的影响  

Effect of polyi:c on angiogenesis in mouse prostate carcinoma tissue

作　　者：田原僮[1] 钟毅 曾靖[1] 赵雪俭[3] 赵丽娟[3] 

机构地区：[1]赣南医学院药理学教研室,江西赣州341000 [2]江西省赣县人民医院泌尿外科,江西赣县341100 [3]吉林大学基础医学院病理生理学教研室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2009年第6期1029-1031,I0011,共4页Journal of Jilin University：Medicine Edition

基　　金：科技部国际科技合作重点项目资助课题(2004DFB02000)

摘　　要：目的:探讨聚肌胞对小鼠前列腺癌组织内血管生成的影响,阐明其可能机制。方法:将16只C57BL6/J纯系荷瘤小鼠按瘤重随机分为对照组和聚肌胞组,分别于瘤内注射生理盐水和聚肌胞(1次·3d-1),用药7次后切除瘤灶,计算瘤重抑制率和瘤指数。肿瘤组织HE染色,镜下观察前列腺癌组织形态学变化及血管分布情况。肿瘤组织血管内皮生长因子(VEGF)、内皮型一氧化氮合酶(eNOS)蛋白免疫组织化学染色,观察其蛋白表达情况。结果:聚肌胞组小鼠前列腺癌的瘤重抑制率为67.85%,瘤指数为(5.42±0.17)%;对照组瘤指数为(14.45±1.06)%,两组比较差异有显著性(P<0.01)。聚肌胞组和对照组前列腺癌组织中VEGF阳性染色肿瘤细胞计数占同类肿瘤细胞的比例分别为(9.64±2.90)%和(37.91±7.62)%,eNOS阳性染色肿瘤细胞计数占同类肿瘤细胞的比例分别为(22.00±8.07)%和(54.43±10.39)%,两组比较差异均有显著性(P<0.01)。结论:聚肌胞通过降低小鼠前列腺癌组织中VEGF和eNOS蛋白的表达,抑制小鼠前列腺癌组织内血管的生成,减少肿瘤组织供氧和养分,对小鼠前列腺癌产生间接抑制作用。Objective To investigate the effects of polyi：c on angiogenesis in mouse prostate carcinoma tissue and their possible mechanisms.Methods Mouse prostate carcinoma models were randomly divided into two groups according to tumor weight：control group and polyi：c group.After treatment for 7 times,the mice were sacrificed and the tumor tissues were cut for weighing,calculating the tumor inhibitory rate and tumor index.Hematoxylin-eosin staining and immunohistochemical staining were performed to observate the morphological changes of prostate carcinoma tissues,distribution of vasa and expressions of vascular endothelial growth factor （VEGF） and endothelial nitric-oxide synthase （eNOS）.Results In polyi：c group,the mean tumor inhibitory rate was 67.85% and the tumor index was（5.42±0.17）%;in control group,the tumor index was（14.45±1.06）%; there was significant differences between polyi：c group and control group（ P〈0.01）. The expressions of VEGF in polyi：c group and control group were （9.64±2.90）% and （37.91±7.62）%,respectively,and there was significant difference between two groups（ P〈0.01）. The expressions of eNOS in polyi：c group and control group were （4.43±10.39）% and （22.00±8.07）%,respectively,and there was significant difference between two groups （ P〈0.01）.Conclusion Through decreasing the expressions of VEGF and eNOS,polyi：c plays an important role in inhibitting the angiogenesis and indirect inhibiting mouse prostate carcinoma.

关 键 词：聚肌胞 前列腺肿瘤 血管内皮生长因子 内皮型一氧化氮合酶 

分 类 号：R73-36[医药卫生—肿瘤]