驱铅益智口服液对铅染毒大鼠的治疗作用  

Lead-expelling effect of Quqian Yizhi Health Oral Liquid in lead-poisoning rats

作　　者：韩长城[1] 于庆海 康维钧[1] 宋波[1] 唐龙妹[1] 

机构地区：[1]河北医科大学公共卫生学院营养与食品卫生教研室,河北石家庄050017 [2]河北省平泉县第二医院,河北承德067500

出　　处：《吉林大学学报（医学版）》2009年第6期1061-1064,共4页Journal of Jilin University：Medicine Edition

基　　金：河北省科技厅科学研究与发展计划项目资助课题(042461600D-4)

摘　　要：目的:探讨驱铅益智口服液对铅中毒大鼠的治疗效果,为预防和治疗铅中毒提供实验室依据。方法:72只SD大鼠随机分为6组:1组为阴性对照组(蒸馏水灌胃),其余5组用2%醋酸铅制备铅染毒模型后分为阳性对照组(蒸馏水灌胃)、EDTA组(0.5%EDTA-2Na腹腔注射)和低、中、高剂量驱铅益智口服液治疗组。干预3周后,断头取血并留取肝脏、肾脏、股骨,采用氢化物发生-原子吸收光谱法测定铅含量,光镜下观察各种组织病理形态学变化。结果:与阳性对照组比较,3种剂量口服液组血液、肝脏、肾脏、股骨组织的铅水平显著降低(P<0.05或P<0.01);3种剂量口服液组血液和肾组织铅水平下降百分率比较差异均无显著性(P>0.05);低剂量口服液组肝组织铅水平下降百分率高于高剂量组和EDTA组(P<0.05或P<0.01);高剂量口服液组股骨组织铅水平显著降低,与低、中剂量口服液组和EDTA组比较差异有显著性(P<0.01)。光镜下观察各脏器组织结构和细胞形态无异常变化。结论:驱铅益智口服液对铅染毒大鼠具有安全、有效的治疗作用。Objective To explore the lead-expelling effect of Quqian Yizhi Health Oral Liquid （QYHOL） in lead-poisoning rats,and provide experimental basis for prevention and therapy of lead poisoning. Methods 72 SD rats were randomly divided into six groups. One group was negative control group only gavaging with distilled water,and others were used to establish lead poisoning models by gavaging 2.0% lead acetate for three weeks.Positive control group was gavaged with distilled water,EDTA group was treated with 0.5% EDTA by abdominal cavity injection and the last three groups were gavaged with low,medium and high-dose QYHOL（1.25,2.50 and 5.00 mg·kg-1 respectively） for three weeks.At the end of the experiment,all rats were killed and the samples of blood,livers,kidneys and femurs were collected.The lead level in each sample was measured by hydride generated-atomic absorption spectrographic method.The pathological changes of tissue sections of liver,kidney and other organs stained with HE were observed by light microscope.Results Compared with positive control group,the lead levels of rat blood samples,livers,kidneys and femurs were markedly decreased after treated with QYHOL with three dosages.Among three dosage QYHOL groups,there were no significant differences in the decreasing percentage of lead levels in blood and kidney tissues （P〉0.05）.The decreasing percentage of lead levels in liver tissues in low-dosage group was higher than those in high-dosage and EDTA groups（P〈0.05 or P〈0.01）.High-dosage QYHOL markedly decreased the lead levels in femur tissues,and compared with low-,mid-dosage and EDTA groups,there were significant differences（P〈 0.01）.All pathological sections with HE staining did not reveal abnormal structures by light microscope.Conclusion QYHOL has safty and effective role on lead-poisoning rats.

关 键 词：铅中毒/药物疗法 大鼠 动物 实验 

分 类 号：R155.33[医药卫生—营养与食品卫生学]