survivin和caspase-9在原发性肝细胞癌组织中的表达及意义  

Expressions of survivin and caspase-9 in human primary hepatocellular carcinoma tissues and their significances

作　　者：王举[1] 窦忠霞[2] 王会春[3] 张文梁[1] 王有德[1] 

机构地区：[1]吉林大学第一医院普通外科,吉林长春130021 [2]吉林省长春市儿童医院呼吸1科,吉林长春130032 [3]内蒙古民族大学医学院

出　　处：《吉林大学学报（医学版）》2009年第6期1119-1122,I0012,共5页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科技发展计划项目资助课题(200705157)

摘　　要：目的:研究survivin和caspase-9在原发性肝细胞癌(HCC)组织中的表达及其与细胞凋亡的关系。方法:应用免疫组化SP法检测42例HCC、42例癌旁组织和12例正常肝脏组织石蜡切片中survivin和caspase-9表达,应用TUNEL法检测细胞凋亡。结果:survivin在正常肝脏组织中无表达,在癌旁组织和HCC中的表达率分别为4.76%和73.81%,二者比较差异有显著性(P<0.01);caspase-9在正常肝脏组织、癌旁组织和HCC中的表达率分别为83.33%、80.95%和47.62%,HCC与前二者比较差异有显著性(P<0.05),而癌旁组织和正常组织比较差异无显著性(P>0.05);survivin和caspase-9在HCC中的表达与患者的年龄、性别、肿瘤大小无明显关系,但与肿瘤组织学分级和转移有密切关联。结论:survivin在HCC中稳定高效表达,可能通过抑制caspase-9而抑制细胞凋亡;survivin高表达和caspase-9低表达可作为HCC独立的不良预后因子。Objective To study the expressions of survivin and caspase-9 in human primary hepatocellular carcinoma（HCC） tissues and their relationship with apoptosis.Methods Immunohistochemical staining was uesed to detect the expressions of survivin and caspase-9 in paraffin sections of 96 cases including 42 HCC tissues,42 paired adjacent noncancerous tissues and 12 normal liver tissues. Apoptosis was detected by TUNEL.Results No immunoreactivity of survivin was seen in normal liver tissues.The positive rates of survivin in HCC and paired adjacent noncancerous tissues were 73.81% and 4.76%,respectively,there was significant difference between two groups（P〈0.01）. The positive rates of caspase-9 in normal tissues,paired adjacent noncancerous tissues and HCC tissues were 83.33%,80.95% and 47.62%,respectively,and the positive rate of caspase-9 in HCC tissues was significantly lower than those in the other two kinds of tissues （P〈0.05）,but the expression of caspase-9 in paired adjacent noncancerous tissues had no difference compared with normal tissues （P〉0.05）.The expressions of survivin and caspase-9 in HCC tissues were not associated with age,sex and the size of tumor,but they were directly associated with tumor histological grade and metastasis.Conclusion High expression of survivin in HCC may inhibit apoptosis through depressing the expression of caspase-9,and it is an indicator of independent poor prognosis.

关 键 词：癌 肝细胞 SURVIVIN CASPASE-9 细胞凋亡 

分 类 号：R735.7[医药卫生—肿瘤]