高效液相色谱流动相手性添加剂法拆分吲达帕胺对映体  

Resolution of Enantiomers of Indapamide by HPLC with Mobile Phase Added with Chiral Agent

作　　者：祝艳琳[1,2] 章靖[1] 田丹碧[1] 

机构地区：[1]南京工业大学理学院,南京210009 [2]无锡吉兴汽车部件有限公司,无锡214000

出　　处：《理化检验（化学分册）》2009年第12期1383-1385,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：以β-环糊精(β-CD)作为手性流动相添加剂,应用反相高效液相色谱研究吲迭帕胺对映体的拆分。在Kromasil C_(18)色谱柱上,采用含12.0 mmol·L^(-1) β-CD,0.05 mol·L^(-1)磷酸二氢钠、2%(体积分数)的三乙胺、pH 2.5的混合溶液-甲醇(75+25)为流动相,流量为0.8 mL·min^(-1),室温下拆分,吲达帕胺对映体可得到良好分离。β-Cyclodextrin （12. 0 mmol· L-1） was used as an additive added to a solution containing 0. 05 mol · L-1 NaHzPO4 and 2~ （by vol. ） of triethylamine （pH 2. 5）, which is mixed with methanol in the ratio of 75 ： 25 and used as the mobile phase in the resolution of enantiomers of indapamide by RP-HPLC. The Kromasil C18 column was used as chromatographic column. Flow-rate of 0. 8 mL ·min-1 was taken for the mobile phase. Under the optimun conditions, the enantiomers of iodapamide were satisfactorily under room temperature.

关 键 词：高效液相色谱 手性流动相添加剂 Β-环糊精 吲达帕胺对映体 拆分 

分 类 号：O657.7[理学—分析化学]