微生物传感器测定河豚毒素研究  

The investigation of biosensor for tetrodotoxin monitoring

作　　者：崔建升[1] 陈婧[2] 王芳[1] 王晓辉[1] 魏复盛[3] 

机构地区：[1]河北科技大学环境科学与工程学院,河北石家庄050018 [2]中国环境管理干部学院科研处,河北秦皇岛066004 [3]中国环境监测总站,北京100029

出　　处：《海洋环境科学》2009年第6期726-728,共3页Marine Environmental Science

基　　金：国家863计划海洋技术领域"卫星遥感大洋渔场环境信息获取技术开发"(2007AA092201);河北省高校重点学科建设项目资助

摘　　要：以地衣芽孢杆菌、假单胞菌和枯草芽孢杆菌为识别元件,采用夹层法固定化微生物膜与氧电极组成毒性微生物传感器,实验了3种不同菌株生物传感器对河豚毒素的响应能力,得出对河豚毒素敏感的微生物菌株为地衣芽孢杆菌。实验得出地衣芽孢杆菌传感器最佳工作条件为pH=7.76,温度35℃,底物GGA质量浓度为21.5 mg/L。在此条件下传感器对河豚毒素响应的标准曲线y=0.0025x＋0.0122,线性范围为10-100μg/mL,相关系数0.9776。Using Bacillus licheniformi,Pseudomonus and Bacillus subtilis as identify element, and they were immobilized by sandwich immobilization, a biosensor was fabricated based on oxygon eletrode. The response properties and optimum measurement for toxicity microbial sensor were investigated. The experiment indicated that, Bicillus licheniformi is sensitity to TTX, the appropriate pH range is 7.8, the optimum temperature is 35℃, and the appropriate concentration of GGA is 21.5 mg/L in bottom fluid. Experiment results indicate toxicity sensor based on Bacillus licheniformis has a linear response from 10 to 100 μg/L and the standary curve of biosensor is y= 0. 0025x ＋ 0. 0122. its linear correlation is 0. 9776.

关 键 词：河豚毒素 微生物传感器 地衣芽孢杆菌 假单胞菌 枯草芽孢杆菌 

分 类 号：Q331[生物学—遗传学]