Tup1突变对转录抑制局部网络中基因表达的非线性影响  

Nonlinear responses in a transcriptional repression local gene network induced by Tup1 gene mutations

作　　者：丛建国 王志伟 任德华[2] 张治洲[1,3] 

机构地区：[1]泰达BIO-X系统生物技术研究中心,天津3000457 [2]计算机科学与信息工程学院天津科技大学,天津300457 [3]哈尔滨工业大学BIO-X海洋生物技术研究中心,威海264209

出　　处：《生物信息学》2009年第4期272-276,共5页Chinese Journal of Bioinformatics

基　　金：教育部新世纪优秀人才计划(2006);国家自然基金(30570401)

摘　　要：研究基因网络的非线性行为特征是研制基因网络技术的基础。Tup1基因是酵母中作用最为广泛的转录抑制因子之一,利用酵母生物信息学数据库中蛋白相互作用关系,构建一个以Tup1为中心,4个层次741个基因的局部基因网络。统计分析六张Tup1不同突变的基因表达芯片数据,将局部基因网络中的全部基因按照3个网络特征进行统计分析:必需\非必需基因、网络层次和网络节点,并研究这些特征与基因表达之间的关系。初步发现基因表达变化的强度与节点数目成一定的反比关系,必需基因的平均变化程度较非必需基因为低,且由Tup1突变引发的其他基因的表达变化在以Tup1为中心的局部基因网络中近层次网络变化程度较大,远层次网络变化程度较低。Characterization of the non - linearity of gene network is a basis for development of gene network technology. Tupl, a global transcriptional repressor of Saccharomyces eerevisiae, was used to construct a four - layer, Tupl - centered local gene network with 741 genes mainly by their protein - interactions. Expression changes of every gene in DNA microarray data based on Tupl deletion and five point mutations were analyzed statistically using parameters of gene essentiality, network layer and node number. It was preliminarily observed that the change scale of gene expression was disproportional to node numbers; the average change scale of essential genes was smaller than non - essential genes;Tupl mutation induced larger expression changes in genes located in network layers with smaller layer number.

关 键 词：局部基因网络 基因表达变化 非线性 Tup1基因 

分 类 号：Q754[生物学—分子生物学]