准噶尔小胸鳖甲抗冻蛋白基因Mpafp149的生物信息学分析  

Bioinformatics Analysis of Antifreeze Protein Gene Mpafp149 from Microdera punctipennis dzungarica

作　　者：邱立明[1] 王艳[1] 李秀明[1] 马纪[1] 

机构地区：[1]新疆生物资源基因工程重点实验室新疆大学生命科学与技术学院,新疆乌鲁木齐830046

出　　处：《生物信息学》2009年第4期314-319,共6页Chinese Journal of Bioinformatics

基　　金：国家自然科学基金(30760056;新疆生物资源基因工程重点实验室开放课题(XJDX0201-200804)

摘　　要：利用生物信息学方法对准噶尔小胸鳖甲抗冻蛋白基因Mpafp149所编码蛋白MPAFP149进行分析,分别从氨基酸组成、理化性质、二三级结构和功能、进化关系等方面进行预测。结果表明MPAFP149与其他昆虫抗冻蛋白在氨基酸水平上具有79%的相似性,二级结构上预测其为水溶性蛋白,含有信号肽和跨膜区。三级结构上与黄粉虫抗冻蛋白YL-1具有较高的相似性,并且具有昆虫抗冻蛋白所特有的结构域。本研究为准噶尔小胸鳖甲抗冻蛋白功能的研究鉴定了基础。Bioinformatics methods were employed to analyze antifreeze protein gene Mpafp149 from Microdera punctipennis dzungarica and its deduced protein MPAFP149 in terms of the amino acid composition, physical and chemical properties, evolutionary relationship, structure and function. The results showed that the protein MPAFP149 encoded by Mpafp149 has 79% of similarity with the other insect antifreeze proteins at the amino acid level. The prediction for the secondary structure of the deduced protein showed it is a water- soluble protein containing the signal peptide and transmembrane region. Its tertiary structure has high similarity with antifreeze protein YL- 1 from Tenebrio molitor, and has unique domain of insect antifreeze proteins. This work laid a foundation for the study of antifreeze protein from Microdera punctipennis dzungarica.

关 键 词：抗冻蛋白 序列比对 结构预测 功能分析 

分 类 号：Q753[生物学—分子生物学]