Fractalkine对大鼠肺动脉平滑肌细胞增殖的影响  

EFFECT OF FRACTALKINE ON PROLIFERATION OF PULMONARY ARTERY SMOOTH MUSCLE CELLS

作　　者：陈小菊[1] 程德云[2] 苏巧俐[2] 樊莉莉[2] 

机构地区：[1]川北医学院附属医院呼吸内科,四川南充637000 [2]四川大学华西医院呼吸内科,四川成都610041

出　　处：《中国应用生理学杂志》2009年第4期445-448,共4页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金资助项目(30370629)

摘　　要：目的:观察fractalkine(FKN)对体外培养的大鼠肺动脉平滑肌细胞(PASMCs)增殖的影响。方法:体外培养大鼠PASMCs,加入不同浓度(10-10、10-9和10-8mol/L)的FKN处理12 h、24 h和48 h,采用四唑盐(MTT)法检测细胞增殖,流式细胞术(FCM)检测细胞周期。结果:MTT试验显示FKN显著促进大鼠PASMCs增殖,此作用呈浓度依赖性。FCM分析显示FKN使S期细胞比例和增殖指数PI值增加。FKN处理PASMCs 12 h后,其S期细胞比例和PI值即出现增加,24 h达高峰。结论:FKN呈浓度依赖方式促进大鼠PASMCs增殖。Aim： To investigate the effect of fractalkine on cell proliferation of cultured rat pulmonary artery smooth muscle cells （PASMCs） in vitro. Methods： Rat PASMCs were cultured in vitro, and treated with different concentrations（ 10-^10, 10^-9, 10^-8 mol/L） of fractalkine for 12 h,24 h and 48 h. The cell proliferation was quantified by MTT assay. The cell cycle of PASMCs was measured by flow cytometric（FCM） analysis. Results： MTT assay showed that fractalkine promoted significantly the proliferation of PASMCs, and the effect was concentration-dependent. FCM analysis indicated that fractalkine increased the percentage of S phase and proliferative index （PI）. The percentage of S phase and PI of PASMCs were increased after treated with fractalkine for 12 hours, which reached a maximal level at 24 hours. Conclusion： Fractalkine promotes rat PASMCs proliferation in a concentration-dependent manner.

关 键 词：FRACTALKINE 肺动脉平滑肌细胞 细胞增殖 

分 类 号：R363[医药卫生—病理学]