纤维蛋白原Bβ-249C/T和Bcl-1G/A基因多态性与脑梗死类型的相关性  

Research on association of beta-fibrinogen gene-249C/T and Bcl-1G/A polymorphisms and type of cerebral infarction

作　　者：杨娜[1] 元小冬[1] 许亚茹[2] 李宏芬[1] 王淑娟[1] 

机构地区：[1]华北煤炭医学院附属开滦医院神经内科 [2]华北煤炭医学院附属医院院内感染科,河北唐山063000

出　　处：《第四军医大学学报》2009年第22期2647-2650,共4页Journal of the Fourth Military Medical University

摘　　要：目的:研究纤维蛋白原(Fg)Bβ-249C/T,Bcl-1G/A的基因多态性、血浆Fg浓度、分子聚合活性等指标与脑梗死(CI)类型的关系.方法:采用病例对照研究方法,选取主干支脑梗死(MCI)患者54例,穿通支脑梗死(PCI)患者106例,正常对照组160例,应用聚合酶链反应-限制性酶切法进行Bβ-249C/T,Bcl-1G/A位点的基因多态性分析.抽取患者清晨空腹静脉血测定血糖等4项生化指标和血浆Fg浓度,Fg单体聚合反应速率(FMPV),最大光密度(Amax),FMPV/Amax等反映Fg分子聚合功能的参数,并进行体检.结果:BβBcl-1G/A的A等位基因和GA,AA基因型在MCI组均高于对照组(P<0.05),FgBβ-249C/T等位基因和各基因型在MCI组,PCI组和对照组组间的分布频率差异无显著性.MCI组Bβ-249C/T的CT+TT型Fg浓度(4.04±0.88)g/L,FMPV(0.72±0.12)均低于CC型Fg浓度(5.01±0.43)g/L,FMPV(0.83±0.06)(P<0.05).对照组BβBcl-1G/A的GA+AA型Fg浓度(4.03±0.90)g/L高于GG型(3.69±0.95)g/L(P<0.05);PCI组BβBcl-1G/A的GA+AA型FMPV(0.74±0.11)高于GG型(0.69±0.11)(P<0.05).结论:Bβ-249C/T基因多态性可能是影响血浆FMPV功能表达的重要位点,BβBcl-1GA,AA基因型可能仅使血浆纤维蛋白单体聚合功能表达增强而诱发PCI.AIM：To explore the relation of Beta-fibrinogen（Fg）gene-249C/T and Bcl-1G/A polymorphisms,the Fg indexes of Fg concentration,molecular polymerized function and the type of cerebral infarction.METHODS：A case-control study was used to analyze 54 patients with main-trunk cerebral infarction（MCI）,106 patients with penetrating cerebral infarction（PCI）and 160 normal control;Gene polymorphisms were analyzed by polymerase chain reaction-restriction fragment length polymorphism;All sample with empty stomach should be drawn venous blood on early morning to determine 4 biochemistry indexes and the parameters illustrated Fg molecular reactivity,such as：Fg concentration,fibrin monomer polymerize velocity（FMPV）,absorbance maximum（Amax）,FMPV/Amax;All sample were examined by trained interviewers.RESULTS：The frequency of A Bcl-1 allele,GA＋AA genotype in the group of MCI were higher than that of normal control（P〈0.05）,No distinction of the frequency of Bβ-249C/T＇s genotype and allele between MCI,PCI and normal control were found.In the group of MCI,Fg concentration and FMPV of Bβ-249C/T CT＋TT genotype were（4.04±0.88）g/L and（0.72±0.12）by turns,while these of CC genotype were（5.01±0.43）g/L and（0.83±0.06）.Fg concentration and FMPV of Bβ-249C/T CT＋TT genotype was lower than that of CC genotype（P〈0.05）.In the group of normal control,Fg concentration of BβBcl-1G/A GA＋AA genotype was（4.03±0.90）g/L,which was higher than GG genotype＇s Fg concentration that was（3.69±0.95）g/L（P〈0.05）.In the group of PCI,FMPV of BβBcl-1G/A GA＋AA genotype was（0.74±0.11）,which was higher than GG genotype＇s FMPV that was（0.69±0.11）（P〈0.05）.CONCLUSION：Bβ-249C/T was probably the important sites of the function express of FMPV,BβBcl-1GA,AA genotype could induce PCI by increasing the function expression of fibrin monomer polymerize function.

关 键 词：纤维蛋白原 基因多态性 Bβ-249C/T BβBcl-1G/A 脑梗死类型 

分 类 号：R743.3[医药卫生—神经病学与精神病学]