MPO对内皮细胞MCP-1表达的影响及二甲双胍的干预研究  

Effect of MPO on monocyte chemo-attractant protein-1 in endothelial cells and the intervention of metformin

作　　者：张栩[1] 于文[2] 管庆波[1] 赵家军[1] 

机构地区：[1]山东大学附属省立医院内分泌科,济南250021 [2]滨州市人民医院内分泌科,山东滨州256606

出　　处：《山东大学学报（医学版）》2009年第11期1-5,共5页Journal of Shandong University:Health Sciences

基　　金：山东省优秀中青年科学家奖励基金项目(2006BS03012);山东省医药卫生科技发展计划项目(2009HW058);2008人事部留学人员科技活动项目择优经费资助

摘　　要：目的观察髓过氧化物酶(MPO)对内皮细胞单核细胞趋化蛋白-1(MCP-1)表达的影响及二甲双胍的干预作用。方法应用western-blot检测内皮细胞内MCP-1、腺苷酸活化蛋白激酶α亚基(AMPKα)蛋白表达,酶联免疫吸附法检测细胞培养液中单核细胞趋化蛋白-1的含量变化。同时应用RNA干扰技术,进一步观察AMPKα的作用。结果MPO显著增加MCP-1的蛋白表达和培养液中MCP-1的浓度(P<0.01),随作用浓度和时间增加而显著增强。二甲双胍显著减少内皮细胞MCP-1的蛋白表达和培养液中MCP-1的浓度(P<0.01),而AMPKαSiRNA干预后,二甲双胍对内皮细胞MCP-1的影响作用显著减弱(P<0.01)。结论MPO可刺激内皮细胞MCP-1的蛋白表达和分泌,二甲双胍通过促使AMPKα磷酸化,激活AMPKα,抑制MPO对内皮细胞的促炎性反应,从而发挥其有益作用。Objective To observe the effect of myeloperoxidase（MPO） on monocyte chemoattractant protein-1（MCP-1） expression in endothelial cells and the intervention of metformin.Methods MCP-1 and AMPKα protein expressions in endothelial cells were determined by the western-blot method,and the level of MCP-1 in culture medium was measured by the sandwich ELISA method.Also,RNA interference technology was used to determine the role of AMPKα.Results Both MCP-1 protein expression in endothelial cells and content of MCP-1 in culture medium were significantly increased by MPO in a dose-and time-dependent manner（P〈0.01）,and metformin decreased MCP-1 protein expression and content of MCP-1 by promoting phosphorylation of AMPKα,however,AMPKαSiRNA significantly weakened the effect of metformin（P〈0.01）.Conclusion Metformin can inhibit the effect of MPO on endothelial cell MCP-1 protein expression and MCP-1 concentration.

关 键 词：髓过氧化物酶 内皮细胞 单核细胞趋化蛋白-1 二甲双胍 腺苷酸活化蛋白激酶α 

分 类 号：R364.5[医药卫生—病理学]