HPLC法测定降糖宁胶囊中格列本脲含量的不确定度分析  

Uncertainty of the content of Glibenclamide in Chinese Traditional Medicine determined by HPLC

作　　者：凌霄 解斐[2] 吴敬德[3] 

机构地区：[1]山东省药品检验所,济南250101 [2]山东大学威海分校海洋学院,山东威海264209 [3]山东大学药学院有机化学教研室,济南250012

出　　处：《山东大学学报（医学版）》2009年第11期124-127,131,共5页Journal of Shandong University:Health Sciences

摘　　要：目的建立高效液相色谱法(HPLC)测定掺入降糖宁中的格列苯脲含量的不确定度评定方法。方法建立数学模型,对含量测定过程中各影响因素进行分析评估。结果通过不确定度来源的识别及量化分析,计算各变量的不确定度和合成不确定度,得出测定结果的扩展不确定度。结论该评定方法适用于HPLC法测定中药中非法掺入的格列苯脲含量的不确定度评定。Objective To establish a method for evaluation of uncertainty of determination of glibenclamide illegally added in Jiangtangning by HPLC.Methods The uncertainty was evaluated by a established mathematical model and various factors affecting sample detection were analyzed.Results Uncertainty of each componet was calculated and their combined uncertainty was then finally obtained by synthesizing the uncertainties of various component variables.Conclusion This method is appropriate to be used in uncertainty evaluation for determination of declofenac sodium in Chinese traditional medicine by HPLC.

关 键 词：高效液相色谱法 格列苯脲 不确定度 

分 类 号：R739.41[医药卫生—肿瘤]