Egfp标记对菌株降解活性与存活能力影响研究  

Effect of egfp Reporter Gene Labeling on Degrading Activity and Survival Ability of Bacteria

作　　者：王海[1,2] 方呈祥[3] 张甲耀[1] 王亚芬[4] 

机构地区：[1]武汉大学资源与环境科学学院,湖北武汉430079 [2]武汉市环境保护科学研究院,湖北武汉430015 [3]武汉大学中国典型培养物保藏中心,湖北武汉430072 [4]中国科学院水生生物研究所,湖北武汉430072

出　　处：《环境科学与技术》2009年第B12期13-16,30,共5页Environmental Science & Technology

基　　金：国家自然科学基金项目资助（20277029）

摘　　要：以蒽为惟一碳源，着重探讨了egfp基因标记对外源降解菌降毹活性和环境竞争能力的影响，研究了石油化工厂污水中存在的混合菌群对外源降解菌的影响。结果表明，egfp基因标记对宿主细胞ETAN1315降解蒽的活性有所降低，但影响甚微，标记菌株仍可用于含蒽废水的处理．EGFP可以作为一种活体指示物，跟踪并实时掌握外源降解菌的生长和运动情况。实验室分离的外源降解菌包括标记的和未标记的，对蒽都具有高效降解活性。与土著混合菌群相比较，外源降解菌显示了环境竞争优势。Anthracene being the sole carbon source in the inorganic substrate, it was studied that egfp reporter gene affected the biodegradability and survival ability of anthracene-degrading strain, it was also studied that consortia, which was isolated from the oil-contaminated sludgy, acted high-efficient bacteria. The result revealed that egfp reporter gene tag could decrease the degradability of strain ETANI315 ,but the effect was so little that labeling strain could still be used to dispose the wastewater containing anthracene. EGFP could be acted as reporter in vivo and be used to trace the real time growth of exogenous degrading mutation. Isolated degrading mutations including labeled and unlabeled strain had the higher-efficient degradability. Meanwhile, compared with consortium, the mutation showed competitive advantage in environment.

关 键 词：egfp报告基因 高效降解菌 降解活性 标记 生物强化 

分 类 号：X172[环境科学与工程—环境科学]