组蛋白去乙酰化酶3及P21蛋白在结肠直肠癌组织中的表达及其临床意义  

Expression of histone deacetylase 3 and P21 in colorectal carcinoma and its relationship with the clinicopathological parameters

作　　者：唐坚[1] 陈锦先[1] 万伯顺[1] 王争[1] 张晞文[1] 

机构地区：[1]上海交通大学医学院附属仁济医院普外科,上海200001

出　　处：《外科理论与实践》2009年第6期639-643,共5页Journal of Surgery Concepts & Practice

摘　　要：目的:观察组蛋白去乙酰化酶3(HDAC3)及P21蛋白在结肠直肠癌组织中的表达及其临床意义;探讨二者间的关系,以及HDAC3与结肠直肠癌临床分期间的关系。方法:以Western印迹法检测30例结肠直肠癌组织及正常黏膜中HDAC3及P21蛋白的表达差异,并分析其与病人临床病理特征间的关系。结果:30例结肠直肠癌组织中HDAC3及P21蛋白的表达与正常黏膜比较有统计学差异(P<0.001);HDAC3及P21的表达与结肠直肠癌的病理分期无关系。结论:HDAC3在结肠直肠癌组织中的表达显著升高,而P21蛋白则明显降低。HDAC3可能通过抑制P21蛋白的表达而引起结肠直肠黏膜的异常增生;二者在结肠直肠癌的发生过程中起较重要的作用。Objective To investigate the expression of histone deacetylase 3 （HDAC3）and P21 in colorectal carcinoma, and its relationship with the clinicopathotogieal parameters, and the clinical staging. Methods Western blot was used in the 30 clinical cases to check the difference in expression of HDAC3 and P21 protein in the colorectal carcinoma tissue and in the neighboring normal mueosa, and also its relationship with the clinicopathologieal parameters was analyzed. Results There was an obvious difference between the expression of HDAC3 and P21 in the colorectal carcinoma proper and in the neighboring normal mucosa, which was statistically meaningful based on semi-quantity analysis （both P=0.000）. The expression level of HDAC3 had no relationship with the pathological staging of the colorectal carcinoma. Conclusions The fact that the expression of HDAC3 increased significantly in eolorectal careinoma while that of P21 dropped dramatically indicates： abnormal hyperplasia of colonic mucosa might be caused by HDAC3 inhibiting the expression of P21. Both HDAC3 and P21 play significant roles in the development of eolorectal carcinoma.

关 键 词：结肠直肠肿瘤 组蛋白脱乙酰基酶 蛋白质P21 

分 类 号：R735.3[医药卫生—肿瘤]