TGF-β1在上皮性卵巢癌及细胞外基质中的表达及意义  

The expression and clinical significance of transforming growth factor-β1 in the epithelial ovarian carcinoma cell and extracellular matrix

作　　者：陈克明[1] 魏华[1] 凌晟荣[1] 易村犍[1] 

机构地区：[1]荆州市第一人民医院妇产科,湖北荆州434100

出　　处：《临床肿瘤学杂志》2009年第11期984-988,共5页Chinese Clinical Oncology

摘　　要：目的:探讨转化生长因子β1(TGF-β1)在上皮性卵巢癌细胞浆和细胞外基质中的表达及其意义。方法:应用免疫组化SABC法检测25份正常卵巢上皮组织、10份良性上皮囊肿、72份上皮性卵巢癌石蜡标本中TGF-β1的表达。半定量RT-PCR检测正常成纤维细胞与卵巢癌相关成纤维细胞TGF-β1 mRNA的相对含量。结果:TGF-β1表达在正常卵巢组织与良性肿瘤之间无显著性差异(P>0.05)。卵巢上皮性癌胞浆中TGF-β1阳性表达低,胞外基质中表达高。上皮性卵巢癌细胞浆与胞外基质TGF-β1,表达与病理分级和临床分期均密切相关。在卵巢癌相关成纤维细胞与正常成纤维细胞中TGF-β1 mRNA的相对含量1.027 0±0.053 9和0.713 1±0.018 6,差异有显著性(P<0.05)。结论:TGF-β1在上皮性卵巢癌发生发展过程中起着一定的作用,TGF-β1在细胞外基质的高表达提示其与肿瘤的浸润转移有关。Objective：To detect the expression of transforming growth factor-β1（TGF-β1） in the cytoplasm and extracellular matrix of epithelial ovarian cancers.Methods：With the SABC immunohistochemical method,the expression of TGF-β1 in tumor cytoplasm and extracellular matrix was detected in paraffin sections of 25 normal ovaries,10 benign tissues and 72 epithelial ovarian cancers.The semi-quantitative reverse transcription polymerase chain reaction（RT-PCR） was used to examine the expressional level of TGF-β1 mRNA in normal and ovarian cancer-associated fibroblast.Results：TGF-β1 expression was found in both cytoplasm and extracellular matrix,there was no significant difference between normal ovaries and benign tissues（P〉0.05）.The expression of TGF-β1 in cytoplasm decreased progressively from benign tissues to epithelial ovarian cancers,while its in extracellular matrix increased（P〈0.05）.TGF-β1 increased when the pathologic grade was getting worse and clinical stage was later in the extracellular matrix and decreased in cytoplasm.The positive rate of TGF-β1 in cytoplasm in the low grade group was lower than that in the high and middle grade（P〈0.05）.The postive rate of TGF-β1 in extracellular matrix in the high grade group was significantly lower than that of the other two groups.In cytoplasma,the TGF-β1 expression was obviously higher inⅠ-Ⅱstage than in Ⅲ-Ⅳ stage,while it was lower in extracellular matrix（P〈0.05）.The relative content of TGF-β1 mRNA of nomal and ovarian cancer-associated fibroblast cell was 1.027 0±0.053 9 and 0.713 1±0.018 6,there was a significant difference（P〈0.05）.Conclusion：The expression of TGF-β1 can play an important role in the progression of epithelial ovarian cancers.The high expression of TGF-β1 in extracellular matrix may be related to the invasion and metastasis of epithelial ovarian cancers.

关 键 词：转化生长因子Β1 卵巢癌 细胞外基质 

分 类 号：R737.31[医药卫生—肿瘤]