基于大豆胞囊线虫病抗性候选基因的SNP位点遗传变异分析  

Genetic variation of SNP loci based on candidate gene for resistance to soybean cyst nematode

作　　者：李英慧[1] 袁翠平[1] 张辰[1] 李伟[1] 南海洋[1] 常汝镇[1] 邱丽娟[1] 

机构地区：[1]国家农作物基因资源与遗传改良重大科学工程农业部作物种质资源利用重点开放实验室中国农业科学院作物科学研究所,北京100081

出　　处：《遗传》2009年第12期1259-1264,共6页Hereditas（Beijing)

基　　金：国家自然科学基金项目(编号:30490251;30471096);国家高技术发展计划(863计划)项目(编号:2006AA100104;2006AA10Z164;2006AA10A111);国家"十一五"科技支撑计划项目(编号:2006BAD13B05);国家重点基础研究发展计划项目(编号:2004CB117203)资助

摘　　要：以我国363份栽培和野生大豆资源为材料,对大豆胞囊线虫抗性候选基因(rhg1和Rhg4)的SNP位点(8个)进行遗传变异分析,以期阐明野生和栽培大豆间遗传多样性及连锁不平衡水平差异。结果表明,与野生大豆相比,代表我国栽培大豆总体资源多样性的微核心种质及其补充材料的连锁不平衡水平较高(R2值为0.216)。在栽培大豆群体内,基因内和基因间分别有100%和16.6%的SNP位点对连锁不平衡显著,形成两个基因特异的连锁不平衡区间(Block)。在所有供试材料中共检测到单倍型46个,野生大豆的单倍型数目(27)少于栽培大豆(31),但单倍型多样性(0.916)稍高于栽培大豆(0.816)。单倍型大多数(67.4%)为群体所特有(31个),其中15个为野生大豆特有单倍型。野生大豆的两个主要优势单倍型(Hap_10和Hap_11)在栽培大豆中的发生频率也明显下降,推测野生大豆向栽培大豆进化过程中,一方面形成了新的单倍型,另一方面因为瓶颈效应部分单倍型的频率降低甚至消失。For clarifying the difference of genetic diversity and linkage disequilibrium （LD） level between cultivated （Glycine max （L,） Merr.） and annual wild soybean （Glycine soja Sieb. ＆ Zucc.）, genetic variation pattern of 8 SNP loci developed from soybean cyst nematode resistance candidate genes rhgl and Rhg4 in soybean germplasm were analyzed. The results indicated that G. max population, consisted of cultivated soybean mini-core collection and modern cultivars, had a higher LD levels （R2value is 0.216） than G. soja population. Since 100% of pairwise loci within a gene and 16.6% of pairwise loci between genes were significant in G. max population, two specific LD regions were formed for each gene. A total of 46 haplotypes were detected in 363 soybean germplasm. The population of G, soja had less number of haplotypes and higher haplotype diversity than the population of G. max. Among the 31 population-specific haplotypes, 15 haplotypes were specific for G. soja population. In addition, the frequency of two major predominant haplotypes （Hap 10 and Hap_11） in G. soja population was obviously decreased in G. max population, which might indicate that some new haplotypes were formed and some old haplotypes were lost during the G. max domesticated from G. soja.

关 键 词：栽培大豆 野生大豆 SNP标记 遗传变异 

分 类 号：S565.1[农业科学—作物学] S968.313