胰高血糖素样肽-2对幼鼠肠缺血-再灌注损伤的保护作用  

Protective effects of glucagon-like peptide 2 on intestinal ischemia-reperfusion injury of young rats

作　　者：张敏[1] 胡毓华[1] 张伟[2] 

机构地区：[1]南京医科大学第一附属医院儿科,210029 [2]南京军区南京总医院普通外科研究所

出　　处：《江苏医药》2009年第12期1473-1475,共3页Jiangsu Medical Journal

摘　　要：目的研究胰高血糖素样肽2(GLP-2)对肠缺血-再灌注(I-R)幼鼠肠屏障功能的保护作用。方法采用肠I-R模型,将24只雄性Wistar幼鼠随机均分为假手术(C组)、肠I-R损伤(B组)和肠I-R损伤+GLP-2保护(A组)三组。光镜下观察小肠黏膜形态学改变,检测肠系膜淋巴结的细菌菌落情况、肠黏膜丙二醛(MDA)、血清D-乳酸、内毒素和肿瘤坏死因子α(TNF-α)水平的变化。结果B组肠黏膜损伤最明显,Chiu′s评分、肠系膜淋巴结细菌菌落计数、肠黏膜MDA、血清D-乳酸、内毒素和TNF-α水平均显著高于C组(P<0.01);与B组相比,A组肠黏膜损伤明显减轻,且Chiu′s评分、肠系膜淋巴结细菌菌落计数、肠黏膜MDA、血清D-乳酸、内毒素和TNF-α水平显著降低(P<0.05)。结论GLP-2能够减轻幼鼠肠I-R损伤、保护肠黏膜屏障、减轻细菌/内毒素易位、减少氧自由基和炎性介质的产生和释放。Objective To explore the protective effects of glucagon-like peptide 2 （GLP-2） on intestinal ischemia-reperfusion（I-R）injury of young rats. Methods Using the rat model of intestinal ischemia-reperfusion,24 young male Wista rats were randomly assigned to three groups of sham operation（group C）,I-R（group B） and I-R＋GLP-2（group A） with 8 rats each. Intestinal pathology was examined under light microscope. Bacterial colony of mesenteric lymph node（MLN）, intestinal mucosal malondialdehyde（MDA）, serum D-lactate, endotoxin, and tumor necrosis factoe（TNF）-α were studied. Results The intestinal injury was significant in group B, and Chiu＇s scores, bacterial colony counts of MLN, intestinal mucosal MDA, serum D-lactate, endo;cgxin, and TNF-α were also significantly higher in group B than those in group C （P〈0. 01）, which were significantly lower in group A than those in group B （P〈0. 05 ）. Conclusion GLP-2 attenuates intestinal I-R injury, protects intestinal mueosal barrier, reduces bacterial/endotoxin translocation, inhibits the release of oxygen free radicles and inflammatory eytokines.

关 键 词：胰高血糖素样肽2 肠缺血-再灌注损伤 D-乳酸 细菌易位 

分 类 号：R364[医药卫生—病理学]