VEGF、c-Met、MMP-9在大肠癌中的表达及意义  

Expression of VEGF,c-Met and MMP-9 in Colorectal Cancer and its Significance

作　　者：陈小萍[1] 柯益斌[1] 

机构地区：[1]莆田学院医学院基础部生化教研室,福建莆田351100

出　　处：《川北医学院学报》2009年第6期542-545,共4页Journal of North Sichuan Medical College

摘　　要：目的探讨VEGF、c-Met、MMP-9在大肠癌组织中的表达及意义。方法分别采用RT-PCR法、W estern印记法、免疫组织化学法测定MMP-9、VEGF、c-Met在大肠癌组织及旁边正常组织的表达情况。结果大肠癌组的MMP-9明显高于正常对照组,两组相比较有显著差异(p<0.05)。大肠癌组的VEGF W estern印记光密度值明显高于正常对照组,两组相比较有显著差异(p<0.05)。c-Met表达方面,正常对照组50例标本中,其阳性表达率为12.0%;大肠癌组50例标本中其阳性表达率为74.0%。比较两组的阳性表达率有显著差异(p<0.05)。结论VEGF、c-M et和MMP-9参与了大肠癌临床病情进展和发病机制。Objective To explore the expression of VEGF, c-Met and MMP-9 in colorectal cancer tissue. Methods VEGF, c-Met and MMP-9 were detected respectively using RT-PCR, Western blotting and Immuno-histochemical method in the colorectal cancer tissue and the adjacent healthy tissue. Results MMP-9 optical density value in colorectal cancer group was significantly higher than that in normal control group （p 〈 O. 05）. VEGF in the Western imprint of optical density value of Colorectal Cancer Group was significantly higher than that in the normal control group （p 〈 O. 05）. For the expression of c-Met in the normal control group of 50 specimens, the positive expression rate was 12.0%. Among 50 cases of colorectal cancer specimens, the positive expression rate was 74. 0%, and comparison between two groups was significantly different （p 〈 O. 05 ）. Conclusion The mechanism of colorectal cancer includes the ac- tivity of VEGF, c - Met and MMP-9.

关 键 词：大肠癌 免疫组织化学法 蛋白免疫印迹法 逆转录聚合酶链反应法 

分 类 号：R735.3[医药卫生—肿瘤]