黄芩苷对LPS诱导人牙髓细胞TLR4表达及TNF-α分泌的影响  

Effects of Baicalin on Toll-like receptor 4 expression and tumor necrosis factor alpha secretion in human dental pulp cells induced by lipopolysaccharide

作　　者：于淼[1] 雷蕾[2] 谭为霞[2] 钟波[3] 张筱薇[3] 吴堪葵[2] 

机构地区：[1]广州医学院附属口腔医院,广东广州510632 [2]暨南大学医学院口腔系,广东广州510632 [3]深圳市第二人民医院口腔科,广东深圳518035

出　　处：《牙体牙髓牙周病学杂志》2009年第11期632-636,共5页Chinese Journal of Conservative Dentistry

摘　　要：目的:研究脂多糖(LPS)诱导人牙髓细胞(HDPCs)炎症信号受体——Toll样受体4(TLR4)表达、肿瘤坏死因子-α(TNF-α)分泌情况以及黄芩苷对其的影响,并探讨黄芩苷可能的细胞保护作用机理。方法:采用组织块法原代培养人牙髓细胞,免疫组化技术鉴定细胞来源,流式细胞术(FC)与酶联免疫法(ELISA)分别检测TLR4与TNF-α的表达。结果:正常HDPCs微量表达TLR4与TNF-α;100μg/mLLPS诱导HDPCs表达TLR4与TNF-α显著增加(P<0.01);与对照组比较,10μg/mL黄芩苷诱导HDPCs表达TLR4与TNF-α,差异无统计学意义(P>0.05);0.001～20μg/mL黄芩苷均可抑制LPS诱导HDPCs表达TLR4与TNF-α,随着黄芩苷浓度升高,TLR4与TNF-α表达逐渐降低,且不同浓度组间比较,抑制作用有显著性差异(P<0.01)。结论:LPS可能通过TLR4信号受体参与牙髓组织炎症过程。黄芩苷可抑制由LPS诱导的TLR4表达及TNF-α分泌,对LPS引起的牙髓炎可能具有潜在的治疗作用。AIM： To study the effects of baicalin on the expression of Toll - like receptor 4 （TLR4） and the secretion of tumor necrosis factor alpha （TNF - α） in human dental pulp cells （HDPCs） induced by lipopolysaccharide （LPS）, and the mechanism of the effects. METHODS： HDPCs were primarily cuhured in vitro and their phenotype was identified by immunohistochemcial staining. Flow Cytometry（FC） was used to analyse the expression of TLR4, and enzyme -labeled innnunosorbent assay （ELISA） was used to measure the secretion of TNF -α. RESULTS ：TLR4 and TNF - α were detected weakly in normal HDPCs , but their expessions significantly increased after being stimulated with LPS at 100μg/mL（P 〈 0.01 ） . The expressions of TLR4 and TNF - α had no statistical differences between the control group and the group of 10μg/mL baicalin （ P 〉 0.05 ）. Baicalin at the concentrations from 0.001 μg/mL to 20μg/mL could significantly inhibit the activity of TLR4 and TNF - α induced by LPS in a dose - dependent manner. Among the different baicalin concentration groups , the differences of expression were statistically significant （P 〈 0.01 ）. CONCLUSION： LPS may participate in the inflammation of pulp tissue by TLR4. Baicalin may play a role in the treatment of pulpitis induced by LPS with the help of inhibiting the expression of TLR4 and secretion of TNF - α.

关 键 词：黄芩苷 脂多糖 人牙髓细胞 TOLL样受体4 肿瘤坏死因子α 

分 类 号：R780.2[医药卫生—口腔医学]