5-脂氧合酶激活蛋白基因和白介素-1A基因多态与缺血性脑卒中的相关性  

Association of polymorphisms of 5-1ipoxygenase activating protein and interleukin-lA genes with ischemic stroke

作　　者：徐佳亮[1] 何志义[1] 李楠[1] 邓淑敏[1] 刘芳[1] 秦雪[1] 李蕾[1] 

机构地区：[1]中国医科大学附属第一医院神经内科,沈阳110001

出　　处：《中华神经科杂志》2009年第12期803-807,共5页Chinese Journal of Neurology

基　　金：辽宁省教育厅2006--2007年度高等学校科研项目计划资助项目（20060981）

摘　　要：目的探讨中国北方地区汉族人群5-脂氧合酶激活蛋白（ALOX5AP）基因和白介素-1A（IL-1A）基因多态与缺血性脑卒中（Is）的相关性。方法采用病例一对照研究，检测构建ALOX5AP基因单倍型HapA的4个位点：SG13S25、SG13S32、SG13S89、SG13S114和IL-1A基因一889位点在对照组、IS组及IS亚组中多态的分布。结果ALOX5AP基因SG13S114位点AA基因型可能是血栓性脑梗死独立的风险因素（OR=1．479，95％CI1．024～2．135，P=0．037），且其风险性主要来源于A等位基因（OR=1．313，95％CI1．017—1．693，P=0．036）；IL-1A基因-889位点T等位基因可能是血栓性脑梗死的易患等位基因（OR=1．540，95％CI1．075～2．204，P：0．023）。HapA单倍型和IS没有相关性，GCGA单倍型可能是Is的危险单倍型（OR=1．683，95％CI1．138—2．487，P=0．008）。同时携带ALOX5AP基因GCGA单倍型和IL-1A-889T等位基因的个体患IS的风险性显著增加（OR=1．608，95％CI1．607～2．423，P=0．022）。结论ALOX5AP基因、IL-1A基因的多态与IS具有相关性，二者的协同作用可显著增加IS的患病风险。Objective To investigate the association of polymorphisms of 5-1ipoxygenase activating protein （ALOXSAP） gene and interleukin-lA （IL-1A） gene with ischemic stroke （IS） in Han population in northern China region. Methods Four SNPs of SG13S25, SG13S32, SG13S89, SG13Sl14 in HapA haplotype of ALOXSAP gene and a SNP （nucleotide 889） at the promoter region in IL-1A were genotyped in control group, IS group and IS subgroups by using PCR-RFLP and MALDI-TOF methods. Results SG13Sl14 AA genotype in ALOXSAP gene indicated to be an independent risk factor of thrombotic cerebral infarction （ OR = 1. 479,95 % CI 1. 024--2. 135, P = O. 037）, and A allele at this SNP particularly showed a correlation with the risk （ OR = 1. 313, 95% C1 1. 017--1. 693, P =0. 036）. IL-1A - 889 T allele might be a susceptible allele related to thrombotic cerebral infarction （OR = 1. 540, 95% C1 1. 075--2. 204,P = 0. 023）. There was no correlation of HapA haplotype of ALOXSAP with IS but G-C-G-A haplotype might be an ＂at-risk＂ haplotype of IS （ OR = 1. 683,95 % CI 1.138--2. 487, P = 0. 008 ）. The individuals who carried ALOXSAP G-C-G-A haplotype and IL-1A - 889 T allele showed significantly increased risk of IS （ OR = 1.608,95% CI 1.607--2.423,P=0.022）. Conclusion ALOXSAP and IL-1A gene polymorphisms correlate with IS and they can have synergistic effect to confer higher risk for IS.

关 键 词：脑缺血 卒中 载体蛋白质类 膜蛋白质类 白细胞介素1Β 多态性 单核苷酸 

分 类 号：R743.3[医药卫生—神经病学与精神病学]