检测日本血吸虫病胶体碳试纸条的研制与初步应用(英文)  

Preparation and preliminary application of colloidal carbon dipstick for Schistosomiasis Japonica

作　　者：汪世平[1] 余路新[1] 车宏莉[1] 陈秀春[1] 秦永华[1] 高冬梅[1] 刘明社[2] 

机构地区：[1]中南大学湘雅医学院基础医学院寄生虫学系,长沙410078 [2]长治医学院寄生虫学教研室,山西长治046000

出　　处：《中南大学学报（医学版）》2009年第11期1063-1069,共7页Journal of Central South University ：Medical Science

基　　金：supported by the Major State Basic Research Development of China(2007CB513108);special program of Hunan11th five-year,P.R.China(2006SK1001);the Key Research Project of Hunan,P.R.China(2007-985-2)

摘　　要：目的:开发一种快速检测血清中日本血吸虫抗体的免疫诊断方法。方法:用胶体碳标记日本血吸虫成熟虫卵可溶性抗原(SEA),与待测血清中日本血吸虫抗体结合,再用SEA包被在硝酸纤维素膜(NC膜)上以捕捉碳标SEA-血吸虫抗体复合物,达到检测血吸虫抗体的目的。结果:检测137份血清,与间接血凝法(indirect haemagglutination assay,IHA)检测结果一致性为98.54%,如经IHA试剂盒检测呈阳性者即视为阳性血清,则该方法的检测敏感性为98.99%,特异性为97.37%;且检测条带的灰度与血清效价具有曲线关系,因而可以对待测血清作半定量分析。结论:胶体碳试条层析法操作简便,快速,具有较高的敏感性和特异性,适合作血吸虫现场检测。Objective To develop a rapid and simple immunoassay to detect antibodies in the sera of patients infect Schistosoma Japonicum （S. Japonicum）. Methods Soluble egg antigen （SEA） of S. Japonicum conjugated with colloidal carbon in advance was used to react with the antibodies in the sera of patients with Schistosomiasis. Then the carbon-antigen-antibody complex would be captured by SEA which had been absorbed on the nitrocellulose membrane and a gray band was shown. Results A total of 137 sera samples from S. Japonicum epidemic area were tested, and the consistency, sensitivity, and specificity of colloidal carbon dipstick assay were 98.54%, 98.99% , and 97.37% , respectively, compared with the IHA method. The gray scale of bands on the dipstick was curvilinear to serum titer which revealed that the assay could be used semi-quantitatively in serum analysis. Conclusion Colloidal carbon dipstick assay is not only rapid and simple, but also sensitive and specific for the detection of serum antibodies of Schistosomiasis Japonica. It will be a practical immunological assay for the diagnosis of Schistosomiasis in the field testing.

关 键 词：日本血吸虫病 胶体碳试纸条 抗体检测 可溶性虫卵抗原 

分 类 号：R446.6[医药卫生—诊断学] R532.21[医药卫生—临床医学]