FGF-2和osteopontin在非小细胞肺癌中的表达及其相关性  

Expression of FGF-2 and osteopontin in non-small cell lung cancer

作　　者：李婷[1,2] 周建华[1] 邓征浩[1] 傅春燕[1] 蒋海鹰[1] 高振芹[1] 王金胜[2] 任宏政[1] 王鹏[1] 

机构地区：[1]中南大学湘雅医学院病理学系,长沙410013 [2]长治医学院,山西长治046000

出　　处：《中南大学学报（医学版）》2009年第11期1114-1119,共6页Journal of Central South University ：Medical Science

基　　金：湖南省自然科学基金(062098)~~

摘　　要：目的:探讨成纤维细胞生长因子-2(fibroblast growth factor-2,FGF-2)和骨桥蛋白(osteopon-tin,OPN)在非小细胞肺癌(non-small cell lung cancer,NSCLC)组织中的表达及其相关性。方法:应用免疫组织化学SP法分析76例NSCLC组织及15例正常肺组织中FGF-2和OPN蛋白的表达水平。采用RT-PCR和Western印迹检测FGF-2对人肺腺癌细胞株A549中OPN mRNA及蛋白表达水平的影响。结果:NSCLC组织中FGF-2和OPN的阳性率分别为65.8%(50/76)和60.5%(46/76),显著高于正常肺组织13.3%(2/15)和0(0/15)(P<0.01),FGF-2和OPN的阳性表达与淋巴结转移、临床分期密切相关(均P<0.01),与年龄、性别和病理分型无明显相关(均P>0.05)。两者表达在NSCLC中呈正相关(r=0.552,P<0.01)。FGF-2可以明显上调A549细胞中OPN mRNA和蛋白的表达(P<0.05)。结论:FGF-2和OPN的高表达与NSCLC的发生及侵袭转移有关,FGF-2的促癌机制可能部分通过上调OPN的表达。Objective To investigate the expression of fibroblast growth factor-2 （FGF-2） and osteopontin （OPN） in non-small cell lung cancer （NSCLC） tissues and analyze the correlation between FGF-2 and OPN. Methods Immunohistochemical SP method was used to detect the expression of FGF-2 and OPN in 76 patients with NSCLC and 15 normal lung tissues. The effect of FGF- 2 on OPN expression at mRNA and protein level in A549 cell was examined by RT-PCR and Western blot. Results The positive expression of FGF-2 （65. 8% ） and OPN （60. 5% ） in the NSCLC tissues was significantly higher than that in the normal lung tissues （ 13.3 % and 0, respectively ） （ P 〈 0.01 ）. The expression of FGF-2 and OPN was closely related to TNM stages and the lymph node metastasis （ all Ps 〈 0.01 ） , but not to histological types, sex, and age of NSCLC patients （ all Ps 〉 0.05 ） . A positive correlation was found between the expression of FGF-2 and OPN in NSCLC （ r = 0. 552 ,P 〈 0.01 ）. The expression of OPN protein and mRNA was up-regulated by FGF-2 in A549 cells. Conclusion The overexpression of FGF-2 and OPN is related to the metastasis and invasion of NSCLC. FGF-2 may promote the metastasis and invasion of NSCLC depending on the upregulation of OPN expression.

关 键 词：FGF-2 骨桥蛋白 非小细胞肺癌 

分 类 号：R734.2[医药卫生—肿瘤]