培养基及培养条件对Pycnoporus sp. SYBC-L1分泌漆酶的影响  

Effect of Fermentation Medium and Conditions on Laccase Production by Pycnoporus sp. SYBC-L1

作　　者：王志新[1] 蔡宇杰[1] 李颜颜[1] 廖祥儒[1] 张峰[1] 张大兵[2] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214122 [2]江苏汉邦科技有限公司,江苏淮安223001

出　　处：《食品与生物技术学报》2009年第6期832-839,共8页Journal of Food Science and Biotechnology

基　　金：长江学者和创新团队发展计划项目(IRT0532)

摘　　要：从作者所在实验室保存的3株白腐真菌中筛选到一株液态发酵产漆酶的密孔菌Pycnop-orus sp. SYBC-L1,以漆酶活力为指标,采用正交试验法,优化了Pycnoporussp.SYBC-L1分泌漆酶的培养基:麸皮水煮液60 g/L,葡萄糖60 g/L,豆粕粉15 g/L,CuSO4.5H2O 1.0 mmol/L;培养条件:初始pH 3.0,装液量50 mL/250 mL,30℃、200 r/min培养13 d,漆酶活力达24.95 U/mL,为优化前的36.16倍。In this study, the nutritional and environmental conditions of a laccase produce strain, Pycnoporus sp. SYBC-L1, were investigated and optimized though single factor experiments and orthogonal experiments. The optimum conditions described as follows： wheat bran cooking liquor 60 g/L, glucose 60 g/L, soybean powder 15 g/L and CuSO4 · 5H2O 1.0 mmol/L, pH 3.0, temperature 30℃, and culture volumn 50 mL/250 mL,respectively. With those optimum conditions, the laccase activity reached at 24.95 U/mL after 13 days culture.

关 键 词：密孔菌SYBC-L1 漆酶 发酵 优化 

分 类 号：Q93[生物学—微生物学]