损伤性窒息诱导癫痫的实验研究  

The study of epllepsy induced by traumatic asphyxia

作　　者：王桂松[1] 张佳梦[1] 郭辉[1] 徐纪文[1] 罗其中[1] 

机构地区：[1]上海第二医科大学附属仁济医院神经外科

出　　处：《功能性和立体定向神经外科杂志》1998年第3期17-18,共2页

摘　　要：目的：研究损伤性窒息致癫痫（ＥＰ）发生的神经细胞形态学改变，以探索ＥＰ发生的机制。方法：通过损伤性窒息ＥＰ的动物模型建立，观察损伤后ＥＰ发生率，神经细胞超微结构变化，海马神经元密度等分析与ＥＰ发作有关的神经细胞形态学改变及相互关系。结果：损伤性窒息后２４小时后ＥＰ发生率为４６．２％（Ｐ＜０．０５）。发作形式：主要为狂奔、全身性痉挛及局限性痉挛。神经细胞超微结构改变显示，除线粒体肿胀外，还有细胞固缩，胞浆浓缩，胞膜内陷，染色体呈块状集聚，细胞泡状改变及凋亡小体形成等凋亡特征。损伤后有ＥＰ发作比无ＥＰ发作者改变更为多见。海马ＣＡ１区神经元细胞密度分析显示，损伤性窒息可导致海马ＣＡ１区神经元密度减少（Ｐ＜０．０５）。结论：损伤性窒息引起脑损伤可诱发ＥＰ，而ＥＰ反复发作又加重神经细胞损害的程度和范围，两者互为因果，提示中断初始的ＥＰ发作。The purpose of the study is to research the mechanism of epilepsy after traumatic asphyxia. Methods: The epilepsy was induced by traumatic asphyxia. The rate of epilepsy was counted, the neuronal ultramicrostructure was observed by electronic microscopy and the neuronal densities was analyzed by computer. Results: The rate of epilepsy after 24hr post asphyxia was 46.2% ( P<0.05 ). The electronic microscopy observation showed characteristic as followed: chromatin densation and marginizationl cell shrinkage, nuclear framentation. apoptotic bodies formation etc. The neuronal densities analysis showed that ND decreased in asphyxia with epilepsy ( P<0.05 ). Conclusion: These results indicated that asphyxia induced epilepsy and epilepsy increased the neuronal deficits.

关 键 词：损伤性窒息 癫痫 形态学 

分 类 号：R742.102[医药卫生—神经病学与精神病学]