双水相体系萃取分离杜仲黄酮  

Extraction and Separation of Flavonoids from Leave of Eucommia ulmoides in Aqueous Two-phase System

作　　者：彭胜[1] 彭密军[1] 黄美娥[1] 卜晓英[1] 伍钢[1] 

机构地区：[1]吉首大学林产化工工程湖南省重点实验室,湖南张家界427000

出　　处：《中药材》2009年第11期1754-1757,共4页Journal of Chinese Medicinal Materials

基　　金：湖南省教育厅重点项目(08A056);吉首大学研究生科研创新项目(JGY200907)

摘　　要：目的:研究双水相体系萃取分离杜仲黄酮的工艺条件。方法:以高分子化合物聚乙二醇4000(PEG4000)/葡聚糖40000(D40)为双水相体系,考察了萃取体系相图,研究了PEG4000/D40质量分数、样品溶液加入量、pH值和温度等因素对双水相成相及杜仲黄酮萃取效果的影响。结果:最佳萃取工艺条件是采用质量分数为11%的PEG4000和质量分数为8%的D40组成双水相体系,加入杜仲黄酮含量为6.85%的样品溶液8 g,控制温度为60℃、溶液pH为8,此条件下杜仲黄酮有较高的萃取率,可达到75.82%。结论:该方法操作条件温和,无溶剂残留,耗时少,对杜仲黄酮的单级分离提纯效率高。Objective： To optimize the aqueous two-phase extraction and separation technology of flavonoids from the leaves of Eucommia ulmoides.Methods： The system was prepared by dissolving suitable amounis of polyethylene glycol 4000（PEG4000） and dextran 40000（D40） in aqueous solution.Phase map of the system,the mass fraction of PEG4000 and D40,the effects of amount of added sample,pH value and temperature on target compounds were investigated systematically.Results： The optimum extraction conditions were PEG4000 11%,D40 8%,8 g sample which contains 6.85% of flavonoids,temperature 60 ℃ and pH value 8.The highest extraction rate could reach to 75.82%.Conclusion： This method has the advantages of mild conditions,solvent-free residues and less time-consumed,and the obvious efficiency in single-stage separation and purification of flavonoids.

关 键 词：黄酮 杜仲 双水相体系 萃取 分离 

分 类 号：R284.2[医药卫生—中药学]