酵母菌半连续转化人参皂苷Rb1的条件优化  

Optimization of fed-batch transformation process of ginesenoside Rb1 by yeast

作　　者：贾翠英[1] 张玉辉[1] 贾斌[1] 

机构地区：[1]河南科技学院,新乡453003

出　　处：《生物学杂志》2009年第6期41-44,共4页Journal of Biology

基　　金：河南省科技攻关项目(07210222004)

摘　　要：以单因素实验为基础,通过多因素方差分析实验对人参皂苷半连续转化的条件进行优化,选出最佳条件组合,得到最佳的补料方式,补料浓度为6%,补料体积为24mL,补料周期为12h,在此条件下人参皂苷Rb1生物转化达33.5%左右。在最佳补料条件下进行人参皂苷酵母菌转化,其稳定性好,转化率高,对工业生产有积极的推动作用。Based on the single-factor experiment, the condition of fed-batch of ginsenoside biotransformation by yeast was studied by multi-factor experiment in order to improve biotransformation efficiency of ginsenoside. The optimal operation conditions of fed-batch process of yeast transformation were obtained as follow： feeding concentration 6 % , feeding volume 24 mL and feeding cycle 12 h. Under the optimal operation conditions, about 33.5 % biotransformation yield was obtained. And also yeast showed steady biotransformation ability and high biotransformation efficiency. Result showed that this method could energetically improve ginsenoside industrial production.

关 键 词：人参皂苷RB1 半连续转化 酵母菌 

分 类 号：Q814[生物学—生物工程]