食源性肥胖对大鼠免疫功能的影响  

Effect of diet-induced obesity on immune function in rats

作　　者：魏中南[1] 王启明[1] 蔡滢[1] 田德润[1] 

机构地区：[1]天津医科大学人体解剖学教研室,天津300070

出　　处：《天津医科大学学报》2009年第4期539-542,共4页Journal of Tianjin Medical University

基　　金：国家自然科学基金资助项目(30870791);天津市应用基础研究计划项目(07JCDJC08100)

摘　　要：目的:利用食源性肥胖(DIO)大鼠模型研究肥胖对脾脏CD4+和CD8+T淋巴细胞含量的影响,并探讨其是否与脾细胞内JAK/STAT通路相关联。方法:(1)通过高能饲料喂养建立DIO大鼠模型。(2)利用流式细胞术检测大鼠脾脏CD4+和CD8+T淋巴细胞含量。(3)利用Western blot检测大鼠淋巴细胞长型的leptin受体(Ob-Rb)以及活化的STAT3蛋白的表达。结果:(1)高能饲料喂养的大鼠体重高于普通饲料喂养大鼠,发育为DIO大鼠。(2)DIO大鼠脾脏CD8+T淋巴细胞含量高于肥胖抵抗(DR)组以及对照组的大鼠,CD4+/CD8+细胞比值低于DR组以及对照组的大鼠。(3)各组大鼠脾脏淋巴细胞Ob-Rb以及活化的STAT3蛋白的表达没有统计学差异。结论:DIO大鼠脾脏CD8+T淋巴细胞含量高于非肥胖大鼠,CD4+/CD8+细胞比值低于非肥胖大鼠,其处于免疫抑制状态;这一变化与DIO大鼠脾脏淋巴细胞的Ob-Rb-STAT3信号转导通路并没有直接的关系。Objective： To study the influence of obesity on splenic CD4^＋ and CD8^＋ T-lymphocytes, and observe the relationship between obesity and the JAK/STAT pathway of splenic lymphocyte. Methods： （1） DIO rat model was established by feeding high-fat diet. （2） Flow-cytometry was used to detect splenic CD4^＋ and CD8^＋ T-lymphocytes of rats. （3） Western blot was used to study the expression of the long signaling isoform of leptin receptor （Ob-Rb） and phospho-stat3 （Tyr705）. Results： After high-fat diet feeding, rats developed diet-induced obesity; body weight of DIO rats was greater than that of the control rats. The number of DIO rats＇ splenic CD8^＋ T-lymphocytes was increased significant than that of DR and lean rats＇, and DIO rats＇ ratio of splenic CD4^＋/CD8^＋ lymphocytes was decreased than that of DR and lean rats＇. Expression of Ob-Rb and phospho-stat3 （Tyr705） had no difference among control, DR and DIO group （P〉0.05）. Conclusion： DIO rat has more splenic CD8^＋ T-lymphocytes and less ratio of splenic CD4^＋/CD8^＋ lymphocytes, DIO rat exhibits an immune inhibition response,and there is no relation between this change and Ob-Rb- STAT3-signaling transduction pathway.

关 键 词：食源性肥胖 LEPTIN T淋巴细胞 LEPTIN受体 STAT3 大鼠 

分 类 号：R589.2[医药卫生—内分泌]