流式细胞仪法检测4HPR诱导Hela细胞凋亡的百分率  

The percentage of 4HPR induced Hela cells apoptosis detected by flow cytometer

作　　者：邵世和 李咏梅 陈曦 孙兰林 李薇 

机构地区：[1]吉林医学院微生物教研室,吉林132001 [2]吉林医学院专科医院 [3]吉林医学院寄生虫教研室

出　　处：《吉林医学院学报》1998年第2期16-17,共2页

基　　金：科委资助

摘　　要：目的：实验结果表明４ＨＰＲ抑制Ｈｅｌａ细胞生长的机理之一是诱导该细胞凋亡。本文用流式细胞仪检测不同浓度的４ＨＰＲ作用不同时间Ｈｅｌａ细胞凋亡的百分比及细胞周期变化。方法：体外细胞培养及流式细胞仪检测法。结果：２μｇ／ｍ的４ＨＰＲ作用２４、４８、７２ｈ后，Ｈｅｌａ细胞的凋亡百分比分别为４．１％、６．６％、２６％；４μｇ／ｍｌ的４ＨＰＲ分别为５．１％、８．３％、２８％、８ｕｇ／ｍｌ的４ＨＲ分别为５．Objective:Experimental results showed that one of the mechanisms of 4HPR inhibit Hela cells is to induce apoptosis.Flow cytometer was used to detect the percentage and cell cycle changes of different concentration 4HPR acted on different stages Hela cells apoptosis.Method:Cell culture in vitro and flow cytome ter detectation.Results:After 24,48,72h acted by 2ug/ml 4HPR,the APO peroentage of hels celle were 4.1%,6.6%;by 4HPR were 5.1%,8.3%,28%; by 8ug/4ml 4HPR were 5.9%, 6.2%, 34%. In the distributions figure of cell cycle,G1 stage cells apparently reduced.Conclusion:The results showedthat the APO percentage of Hela cells was positive correlation with the time and concentration 4HPR action.

关 键 词：流式细胞仪 4HPR 细胞周期 细胞凋亡 百分率 

分 类 号：R73－3[医药卫生—肿瘤]