乳腺导管上皮内瘤变组织中Survivin和CyclinD2的表达及意义  

Expression and clinical significance of Survivin and CyclinD2 in ductal intraepithelial neoplasia

作　　者：刘现军[1] 金东岭[1] 席丰[1] 梁爽[1] 高金生[1] 时志民[1] 

机构地区：[1]河北工程大学医学院病理学教研室,河北邯郸056002

出　　处：《中国妇幼保健》2009年第36期5124-5126,共3页Maternal and Child Health Care of China

基　　金：河北省科技攻关项目[06276102D-55];邯郸市科技研究与发展项目[2005-10906]

摘　　要：目的:探讨Survivin和CyclinD2在乳腺导管上皮内瘤变(DIN)中的表达情况,寻求有助于乳腺导管上皮内瘤变诊断的分子标记。方法:通过免疫组化SP法检测20例普通型导管增生(UDH)、15例DIN1A、30例DIN1B、21例DIN1C-3组织中的Survivin和CyclinD2表达情况,并以正常乳腺组织作为对照。结果:Survivin在UDH/DIN1A、DIN1B和DIN1C-3组中的阳性率分别为0、36.6%和81.0%。DIN1B和DIN1C-3两组比较差异有统计学意义(P<0.001)。CyclinD2在UDH、DIN1A、DIN1B和DIN1C-3组中的表达率分别为10%、13.3%、41.9%和76.2%,UDH、DIN1A与DIN1B和DIN1C-3比较差异有统计学意义(P<0.001),DIN1A与DIN1C-3两组比较差异亦有统计学意义(P<0.001),UDH与DIN1A比较差异无统计学意义(P>0.05)。结论:从正常乳腺组织到UDH、DIN1A、DIN1B及DIN1C-3的恶性转化过程中伴有Survivin和CyclinD2表达的逐渐增高,其过度表达可能是某些乳腺癌发生过程中的早期事件,Survivin和CyclinD2可作为乳腺UDH、DIN1A与DIN1B和DIN1C-3鉴别诊断的标记物。Objective： To explore the expression levels of Survivin and CyelinD2 in ductal intraepithelial neoplasia （ DIN）, search for a molecular marker of duetal intraepithelial neoplasia. Methods： The expression levels of Survivin and CyclinD2 in 20 cases of usual ductal hyperplasia （UDH）, 15 cases of ductal intraepithelial neoplasia 1A （DIN1 A） , 30 cases of ductal intraepithelial neoplasia 1B （DINIB） and 21 cases of ductal intraepithelial neoplasia 1C -3 （DIN1C -3 ） were detected by immunohistoehemistry, and compared with normal breast tissues. Results： The positive rates of Suvivin in UDH/DIN1A, DIN1B and DIN1C - 3 were 0, 36. 6% and 81.0% , respectively. There was significant difference between DIN1B and DINIC -3 （P 〈0. 001 ） . The positive rates of CyelinD2 in UDH, DIN1A, DIN1B and DINIC -3 were t0% , 13.3% , 41.9% and 76. 2% , respectively, there was significant difference between UDH, DIN1A and DIN1B, DINIC -3 （P 〈 0. 001 ）, as well as between DIN1A and DIN1C -3 （P 〈 0. 001 ）, but there was no significant difference between UDH and DIN1A （P 〉0.05） . Conclusion： The expression levels of Survivin and CyclinD2 increase with the transformation from UDH, DIN1A, DIN1B to DIN1C -3, the overexpression of Survivin and CyclinD2 maybe indicators of oncogenesis of breast cancer, which can be regarded as indicators of differential diagnosis of UDH, DIN1A and DIN1B.

关 键 词：乳腺肿瘤 乳腺导管上皮内瘤变 SURVIVIN CYCLIND2 

分 类 号：R737.9[医药卫生—肿瘤]