有效bcl-2siRNA对HL-60细胞生长的抑制作用  

Inhibitation of effective bcl-2 siRNA on apoptosis of human leukemia-60 cells

作　　者：燕春艳[1] 杨庆媛[1] 魏红[1] 雷小勇[2] 涂玉林[3] 王旭[1] 崔文[1] 孔灵玲[1] 

机构地区：[1]济宁医学院病理教研室,272013 [2]南华大学药物药理研究所 [3]南华大学心血管病研究所

出　　处：《白血病．淋巴瘤》2009年第12期712-713,共2页Journal of Leukemia & Lymphoma

摘　　要：目的应用RNA干扰（RNAi）技术观察有效bcl-2siRNA对白血病细胞HL-60凋亡的影响。方法将有效bcl-2siRNA转染入HL-60细胞；用MTT法、流式细胞术（FCM）及Hoechst33258荧光染色法观察细胞增生及凋亡情况。结果有效bcl-2siRNA有抑制HL-60细胞生长及促进细胞凋亡的作用。结论有效bcl-2siRNA能特异性促进HL-60细胞凋亡，抑制其生长。Objective To study the effect of bcl-2 siRNA on apoptosis of HL-60 Ceils. Methods bcl-2 siRNA was synthesized in vitro transcription with silencer siRNA construction kit.The synthesized siRNA was transfeeted into HL-60 cells with Amine siPORT transfection. We used MTT, flow ：cytometer and hoechst 33258 floureseenee stainning to evaluate cell proliferation and apoptosis. Resuits ：Bcl-2 siRNA could partially inhibit the growth of HL-60 cells. After incubated with bcl-2 siRNA1 for 48 hours, HL-60 cells exhibited morphologie characteristic of apoptosis including chromatin condensation, crescents formation and nuclear fragmentation. Conclusion Effective bel-2 siRNA can induce apoptosis and inhibit cell proliferation.

关 键 词：RNA干扰 基因 BCL-2 RNA 双链 HL-60细胞 细胞凋亡 

分 类 号：R733[医药卫生—肿瘤]