大剂量地塞米松预处理对大鼠脑缺血再灌注损伤的保护作用  

Protective effects of large dosage of dexamethasone preconditioning on cerebral ischemia reperfusion injuries in rat

作　　者：金日华[1] 朴莲花[2] 金炳植[3] 赵春植[3] 

机构地区：[1]吉林大学第一医院神经外科,吉林长春130021 [2]日本国立佐贺大学神经生理研究室 [3]延边大学附属医院,吉林延吉133000

出　　处：《吉林医学》2009年第23期2936-2938,共3页Jilin Medical Journal

摘　　要：目的:研究缺血预处理和大剂量地塞米松(dexamethasone,Dex)预处理对大鼠脑缺血再灌注损伤的保护效应,探寻更为有效的脑损伤的防治方法及检测手段。方法:用改良后的线拴法制备大鼠大脑中动脉闭塞(MCAO)模型,用电刺激方法检测大鼠三叉神经皮层诱发电位(TSEP)。结果:随着缺血再灌注损伤的加重,TSEP波幅下降,而潜伏期延长。缺血预处理组和地塞米松预处理组与缺血再灌注组相比,差异均有统计学意义(P<0.01),缺血预处理组和地塞米松预处理组相比差异无统计学意义(P>0.05)。结论:缺血预处理和大剂量Dex预处理能显著减轻脑缺血再灌注损伤,Dex预处理具有良好的防治脑缺血再灌注损伤的作用。TSEP可作为检测脑缺血再灌注损伤及推测神经功能恢复的客观指标。Objective To investigate ischemie preconditioning and protection effect of large dosage of dexamethasone preconditioning on the cerebral ischemia reperfusion injuries in rats, and seek more effective methods of the cerebral protection and detective means. Method The experimental MCAO model was set up with middle cerebral artery occlusion by modification of Longua with suture method, and the trigeminal somatosensory evoked potential （TSEP）was detected with electrostimulation. Results Increasing with ischemia reperfusion injury,the TSEP＇s amplitude was decreased and the latent period was lengthened. The results showed that ischemia preconditioning group and Dex preconditioning group both showed significant difference compared with ischemia - reperfusion group （ P 〈 0. 01 ）. Contrarily, there was no significant difference between ischemia preconditioning group and Dex preconditioning group（ P 〉 0. 05）. Conclusion Ischemia preconditioning and large dosage of dexamethasone preconditioning may alleviate cerebral ischemia reperfusion injuries, and dexametbasone preconditioning has the satisfactory protective effect of the cerebral ischemia repeffusion injuries. TSEP can detect cerebral ischemia reperfusion injuries and provide an objective reliable index of neural functional recovery.

关 键 词：地塞米松 脑缺血再灌注 预处理 诱发电位 

分 类 号：R743.3[医药卫生—神经病学与精神病学] R542.2[医药卫生—临床医学]