3,5-二硝基水杨酸法测定椰子纳塔发酵液中总糖含量研究  

Study on colorimetric determination of water-soluble total sugar in Nata de coco fermentation broth with 3,5- dinitrosalicylic acid

作　　者：黄翊鹏[1,2] 祁静[1,2] 郑亚军[2,3] 赵松林[2,3] 陈卫军[2,3] 李枚秋[2,3] 

机构地区：[1]海南大学食品学院,海南儋州5717372 [2]中国热带农科院椰子研究所,海南文昌571339 [3]国家重要热带作物工程技术研究中心,海南儋州571737

出　　处：《广东农业科学》2009年第12期171-174,共4页Guangdong Agricultural Sciences

基　　金：国家科技支撑项目(2007BAD76B01);公益性行业(农业)科研专项(200903026)

摘　　要：建立了椰子纳塔发酵液中总糖含量的测定方法。探索了吸收光谱、显色剂用量、显色时间、HCl浓度等测试条件。结果表明,波长550 nm处溶液的吸光度与葡萄糖含量有良好的线性关系,线性范围为0.2～11.0 mg/mL,R=0.9994;最佳显色剂量为2 mL,最佳显色时间为2 min,最佳水解总糖的HCl浓度为3 mol/L,样品平均回收率为100.08%(RSD=1.38%,n=5)。说明该方法重复性好,可作为常规分析方法使用。The experiment established a method for the determination of water-soluble total sugar in nata de coco fermentation broth and studied on the optimum conditions of 3,5- dinitrosalicylie acid colorimetry. We discussed the wavelength, chromogenic, dosage, and coloring time an so on, which effected the results. According to the experiment, the optimal testing wavelength,chromogenic dosage, coloring time and HCI concentration were decided to be 550 nm, 2 mL,2 min,3 mol/L, respectively. While at the wavelength of 550 nm, the linear range of glucose was 0.2-0.8 mg/mL （r=0.9994）, the average recoveR, was 100.08%（RSD=1.38%,n=5）. The results indicate that this method with high fidelity, it can be used as a routine method.

关 键 词：3 5-二硝基水杨酸 椰子纳塔 发酵液 总糖 

分 类 号：S39[农业科学—农艺学]