交感神经递质NE对内毒素诱导大鼠kupffer细胞TNF-α/IL-1β表达的影响  

EFFECTS OF NORADRENALIN ON TNF-α AND IL-1β EXPRESSIONS IN RAT KUPFFER CELLS INDUCED BY LPS

作　　者：曾议[1] 夏锋[1] 田峰[1] 程龙[1] 王曙光[1] 

机构地区：[1]第三军医大学附属西南医院全军肝胆外科研究所西南肝胆外科医院,重庆400038

出　　处：《肝胆外科杂志》2009年第6期458-461,共4页Journal of Hepatobiliary Surgery

基　　金：国家自然科学基金资助项目(30772095)

摘　　要：目的探讨交感神经递质去甲肾上腺素对体外培养大鼠kupffer细胞TNF-α、IL-1β炎症相关细胞因子表达的影响。方法分离大鼠kupffer细胞体外分三组培养,经体外培养48 h后,给予外源性内毒素(LPS 10μg/ml)刺激,同时给予不同浓度的去甲肾上腺素(0.1μmol/L^10μmol/L)分别作用12 h,运用定量逆转录多聚酶链反应检测各处理组TNF-α和IL-1β mRNA的表达,ELISA检测细胞培养上清TNF-α IL-1β蛋白的表达。结果同时给予LPS(10μg/ml)刺激和去甲肾上腺素(1μmol/L及10μmol/L)作用后,kupffer细胞培养上清TNF-α和IL-1β蛋白较LPS组显著增高(P<0.05);TNF-α mRNA表达分别较LPS组增加50.9%(P<0.05)和59.1%(P<0.05);IL-1β mRNA较LPS组表达增加53.7%(P<0.05)和57.8%(P<0.05)。结论应激浓度的去甲肾上腺素能增加内毒素诱导大鼠kupffer细胞TNF-α、IL-1β表达,具有促炎效应。Objective To evaluate the effect of noradrenalin on the expression of TNF-α and IL-1βin rat Kupffer ceils induced by lipopolysaeeharide （LPS）. Methods The isolated rat kupffer ceils cultured in vitro divided into three groups, Given lipopolysaceharide （ 10 μg/ml）with or without norepinephrine （0. 1 μmol/L - 10 μmo]/L） respectively for 12 hours. Using quantitative reverse transeription-polymerase chain reaction to detect TNF-α and IL-1β mRNA expression, ELISA detect TNF-α, IL-1β protein expression of cell culture supernatant. Results In the LPS and norepinephrine（ 1 μmol/L and 10 μmol/L）group,the cell culture supematant TNF-α and IL-1β protein were significantly higher than that in LPS group （ P 〈 0. 05 ） ; TNF-α mRNA expression were higher than that in LPS group 50. 9% （P 〈 0.05 ）and 59. 1% （P 〈 0. 05 ）Respectively;IL-1β mRNA expression increased 53.7% and 57. 8% than that in LPS group（ P 〈 0.05 ）. Conclusion The high concentration of noradrenalin could enhance the expressions of TNF-α and IL-1β of hepatic Kupffer cells induced by lipopolysaccharide and has pro-inflammatory effects.

关 键 词：去甲肾上腺素 肝枯否细胞 TNF-a和IL-1βmRNA 内毒素 

分 类 号：R575[医药卫生—消化系统]