葛根素及葛根提取物在大鼠体内药代动力学研究  

Study on pharmacokineics of puerarin and puerarin crude extract in rats

作　　者：李煦颖[1] 赵妍[1] 王琳[1] 张懋[1] 王欣[1] 孟胜男[1] 

机构地区：[1]中国医科大学药学院药剂学教研室,辽宁沈阳110001

出　　处：《中国生化药物杂志》2009年第6期383-386,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：辽宁省自然科学基金资助项目(编号20082103)

摘　　要：目的考察葛根素及葛根提取物在大鼠体内的药代动力学差异。方法大鼠分别灌胃给予葛根素(500mg/kg)或葛根提取物(相当于葛根素500 mg/kg),在不同时间点采血,以对羟基苯甲酸为内标,应用高效液相色谱法测定葛根素血药浓度,采用DAS 2.0软件计算药动学参数。结果葛根素及葛根提取物在大鼠体内药动学过程均符合二室模型,比较二者主要药动学参数:提取物组较葛根素组的AUC0-t、Cmax显著降低,Tmax、t1/2z、CL/F及Vz/F显著增加。结论葛根素及葛根提取物的主要药动学参数具有显著性差异,葛根提取物中复杂成分的存在能影响其有效成分葛根素的体内药动学行为。Purpose To compare pharmacokineics of puerarin and crude extract in rats. Methods Rats received 500 mg/kg puerarin and puerarin crude extract by oral administration respectively. Hydroxybenzoic acid was selected as internal standard and the plasma concentration of the puerarin and crude extract was analyzed by HPLC. The pharmacokinetics parameters were calculated with DAS2.0. Results The pharmacokinetics of puerarin and puerarin crude extract was both best fitted with two-compartment models in rats after oral administration, and the pharmacokinetics main parameters of the two formulations were different ： the AUC0-1 and Cmax of puerarin were much greater than those of puerarin crude extract,but T t1/2z, CL/F and V2/F were much lesser than those of puerarin crude extract. Conclusion The complex components in pueraria crude extract can affect the pharmacokinetics of puerarin in rat in vivo.

关 键 词：葛根素 葛根提取物 药代动力学 高效液相色谱法 

分 类 号：R969.1[医药卫生—药理学]