细胞粘附在结肠癌细胞系SW480多药耐药表型中的作用  

Roles of cell adhesion in multidrug resistance phenotype of colon cancer cell line SW480

作　　者：陆春雷[1] 吴国忠[2] 尹天泉[1] 同李平[1] 丰帆[1] 郑琇山[1] 张洪伟[1] 

机构地区：[1]第四军医大学西京消化病医院消化一科,陕西西安710032 [2]解放军第101医院普通外科,江苏无锡214044

出　　处：《东南国防医药》2009年第6期485-487,490,共4页Military Medical Journal of Southeast China

摘　　要：目的探讨细胞粘附在结肠癌细胞系SW 480多药耐药表型中的作用。方法通过细胞粘附实验、四甲基偶氮唑盐实验(MTT法)、细胞内阿霉素的蓄积和潴留及Annexin V/PI染色法检测凋亡等检测粘附于层粘连蛋白(LN)的结肠癌细胞系SW 480的多药耐药性的改变。结果粘附于LN的SW 480细胞对化疗药物敏感性显著下降,化疗药物的IC50均显著升高,化疗药物诱导的凋亡指数明显减少;细胞内阿霉素的蓄积和潴留均明显减少,药物泵出率显著增加(P<0.05)。结论结肠癌细胞SW 480与LN粘附后,可能提高了对药物转运的能力,导致化疗药物在肿瘤细胞内蓄积的减少;通过提高抗凋亡的能力,逃避化疗药物诱导的凋亡。Objective To explore the roles of cell adhesion in multidrug resistance （ MDR ） of colon eancer cell line SW480. Methods The cell adhesion assay, MTT assay, Adriamycin （ADR） accumulation and retention and Annexin V/PI staining were used to illuminate the drug resistance of colon cancer cell line SW480 after adhesion to laminin （ LN ）. Results After adhesion to LN, SW480 cells exhibited a significantly higher IC50, medicine pumping rate and a reduction of drug sensitivity, apoptosis index induced by ehemotherapeutics and accumulation and retention of ADR （P 〈 0.05）. Conclusion Adhesion to LN may promote MDR phenotype of colon cancer cells by two means： The adhesion of SW480 to LN may promote the drug transport ability and decreases the accumulation of chemotherapeutic drugs in tumor cells;The adhesion may elevate the capability of anti-apoptosis and inhibit the apoptosis induced by chemotherapeutic drugs.

关 键 词：结肠癌 层粘连蛋白 细胞粘附介导耐药 凋亡 

分 类 号：R735.35[医药卫生—肿瘤]