灵芝多糖诱导人肝癌细胞HepG2凋亡的研究  

Research about the apoptosis of liver cancer HepG2 cells induced by Ganoderma lucidum polysaccharides

作　　者：徐晋[1] 吴丽[1] 徐巧芳[1] 

机构地区：[1]江苏省第二中医院外科,江苏南京210017

出　　处：《中国当代医药》2009年第23期7-9,共3页China Modern Medicine

摘　　要：目的:研究灵芝多糖(GLPS)与化疗药氟尿嘧啶联合使用诱导人肝癌细胞HepG2凋亡作用。方法:MTT法测定细胞毒作用,流式细胞术检测细胞凋亡率,比色法测定Caspase-3、Caspase-8酶活性,Western-Blotting法检测细胞色素C、Caspase-3、Caspase-8蛋白表达情况。结果:灵芝多糖与氟尿嘧啶联合使用具有显著的细胞毒作用;流式细胞术检测,随着GLPS浓度增加,细胞凋亡率升高,Caspase-3、Caspase-8酶活性亦增强;Western-Blotting检测发现GLPS作用后细胞色素C、Caspase-3、Caspase-8蛋白表达不同程度增加。结论:灵芝多糖与氟尿嘧啶联合使用可通过引起细胞色素C的释放,活化Caspase诱导肝癌细胞凋亡。Objective：To evaluate the apoptosis of Ganoderma lucidum polysaccharides （GLPS）combine with 5-fluorouracil （5-FU） in HepG2 cells.Methods：MTT was applied to analyze the cytotoxicity with different concentration in HepG2 cells, the flow cytometric analysis was performed to detect apoptosis.Caspase-3 relative activity was determined by colorimetric assay and expression of Cytochrome C,Caspase-3 and Caspase-8 proteins was detected by Westem-Blotting.Results：GLPS combined with 5-FU was significant in HepG2 cells.GLPS treatment resulted in a concentration dependent increase in apoptosis ratio detected by fluorescence activated cell sorter,and activity of Caspase-3 and Caspase-8 were increased,too. Meanwhile expression of Cytochrome C、Caspase-3 and Caspase-8 proteins raised as well.Conclusion：GLPS combine with 5-FU can induce apoptosis in HepG2 cells through inducing release of Cytochrome C then active Caspase passway.

关 键 词：灵芝多糖 肝癌 凋亡 CASPASE 细胞色素C 

分 类 号：R979.1[医药卫生—药品]