两株致病疫霉拮抗真菌复合发酵工艺优化试验研究  

Experimental Study on Mixed Fermentation Conditions Optimization of Two Fungus Against Phytophthora infestans

作　　者：李莎[1] 蒋继志[1] 王会仙[2] 

机构地区：[1]河北大学生命科学学院,河北保定071002 [2]河北大学图书馆

出　　处：《安徽农学通报》2009年第24期20-22,共3页Anhui Agricultural Science Bulletin

基　　金：河北省自然科学基金项目(C2007000191);公益性行业(农业)科研专项:(3-20)

摘　　要：为了使2株致病疫霉拮抗真菌发酵水平达到最佳效果,为其工业化发酵生产和田间应用提供参考,采用滤纸片法通过单因素实验,确定了最佳共培养发酵环境。结果表明,将白菜黑斑病菌1片菌饼先接入含80mL/250mL培养基中,25℃恒温黑暗静置培养24h后再以1:1的比例接入梨黑斑病菌菌饼,复合发酵6d后其抑菌效果最好,抑菌率最高可达87%以上。2种拮抗菌复合发酵后对致病疫霉的抑制活性优于单独发酵。The fermentation conditions for two fimgi against Phytophthora infestans were studied through single factor experiment. The sequence of inoculation of mixed culture of two strains were that Alternaria brassieae was inoculated in the medium and cuhured 24h under 25℃ ,then Alternaria alternata was inoculated and mixed cultured until 6d. It was demonstrated that the favorable fermentation conditions were that the proportion of the two was 1：1, and the inhibitory rate was higher than 87%. The anti-fungi activity against Phytophthora infestans of mixed fermation was better than separated fermation of the two fungus.

关 键 词：致病疫霉 混合发酵 抑菌活性 

分 类 号：Q939.5[生物学—微生物学]