孕小鼠注射海洛因对仔鼠体重、行为及肺中相关活性物质表达的影响  

Behavior,Weight and Expression of KGF,c-Fos and Bax in the Lungs of Filial Mice after Injecting Pregnant Mice with Heroin

作　　者：冯红丽[1] 李重阳[2] 俞诗源[1] 王悦[1] 王昱[1] 贾艳芳[1] 邓海平[1] 郭婷婷[1] 牛宪峰[1] 

机构地区：[1]西北师范大学生命科学学院,兰州730070 [2]甘肃政法学院,兰州730070

出　　处：《动物学杂志》2009年第6期23-30,共8页Chinese Journal of Zoology

基　　金：国家自然科学基金项目(No.30370163);甘肃省自然科学基金项目(No.ZS031-A25-005-Z);甘肃省创新人才基金项目

摘　　要：为了探讨海洛因对仔鼠行为、体重及肺发育中KGF、c-Fos蛋白和Bax蛋白表达的影响,对4组共48例受孕小鼠(Mus musculus)从第8d开始,每天早晚分别注射0.2ml浓度为1.0g/L、1.5g/L和2.0g/L的海洛因溶液和等量的生理盐水直到小鼠分娩,观察测量仔鼠行为及体重变化,采用免疫组织化学染色和体视学半定量方法检测15d胚胎、出生后1d、7d、15d仔鼠的肺中KGF、c-Fos蛋白和Bax蛋白表达情况。结果表明,海洛因影响仔鼠的行为活动,实验组各发育期仔鼠的体重明显低于对照组,海洛因组各发育期仔鼠肺中KGF、c-Fos蛋白和Bax蛋白的表达强度与对照组相比显著增强(P<0.01或P<0.05),且随海洛因浓度的增大表达越强,但随着发育的进行,海洛因组仔鼠肺中KGF、c-Fos蛋白和Bax蛋白的阳性表达减弱。海洛因影响仔鼠的行为与体重,海洛因对各发育期仔鼠肺的损伤可能与肺组织中KGF、c-Fos蛋白和Bax蛋白表达的增强有关。To study the effects of heroin on changes of weight,behavior and expression of KGF,c-Fos and Bax in the lungs of developing filial mice,48 mice at the eighth day of pregnancy were divided into 4 groups and injected with different concentrations （0,1.0,1.5 and 2.0 g/L） of heroin in 0.2 ml saline in the morning and afternoon until the birth of filial mice.Changes of weight and behaviour were observed and expression of KGF,c-Fos and Bax was detected by immunohistochemistry combined with stereological semi-quantitative methods in the lungs of 15-day embryos,and filial mice 1 d,7 d and 15 d after birth.Results showed that heroin influenced behaviour of the developing mice.The weight of filial mice of the experimental groups was lower than that of the control group at different developmental stages,and the expression intensity of KGF,c-Fos and Bax in the lungs of filial mice significantly increased （P〈0.01 or P〈0.05）.However,the expression of KGF,c-Fos and Bax decreased gradually in the lungs of filial mice with the development.Thus,heroin significantly affects the weight and behavior,and damages the lungs in filial mice,the latter might be correlated with the increased expression of KGF,c-Fos and Bax.

关 键 词：海洛因 小鼠 体重 行为变化 KGF C-FOS蛋白 BAX蛋白 

分 类 号：R749.6[医药卫生—神经病学与精神病学]