Foxo3a对胃癌细胞SGC7901凋亡的促进作用  

Apoptosis-promoting effects of forkhead box O3a in human gastric cancer SGC7901 cells

作　　者：谭小平[1] 张庆[1] 朱燕妮[1] 何长华[1] 董卫国[2] 范文[1] 

机构地区：[1]长江大学附属第一医院消化科,湖北省荆州市434002 [2]武汉大学人民医院消化科,湖北省武汉市430060

出　　处：《世界华人消化杂志》2009年第30期3092-3095,共4页World Chinese Journal of Digestology

摘　　要：目的:观察转录因子Foxo3a对胃癌细胞SGC7901凋亡的影响,并初步探讨其促进凋亡的分子机制.方法:构建Foxo3a真核表达质粒,转染培养的胃癌细胞系SGC7901 4-6h,在FBS完全培养基培养24h后,换成0.1%无血清的培养基中培养8h,TUNEL染色观察对胃癌细胞凋亡的影响,Western blot检测切割的caspase-3和PARP水平.结果:成功构建了Foxo3a真核表达质粒.转染胃癌细胞后,与对照组相比,1和2μgFoxo3a处理组胃癌细胞凋亡率达到了5.8%±2.3%和11.1%±3.4%,是对照组的近2和4倍.caspase-3和PARP活性明显增高.结论:Foxo3a是一种肿瘤抑制因子,可通过促进caspase-3和PARP的活性来诱导癌细胞凋亡,从而抑制肿瘤细胞的生长.AIM： To investigate the apoptosis-promoting effects of forkhead box O3a （Foxo3a） in human gastric cancer SGC7901 cells and explore molecular mechanisms involved. METHODS： Foxo3a expression plasmid was introduced into SGC7901 cells via liposomemediated transfection. Eight hours after transfected cells were cultured in culture medium containing 0.1% fetal bovine serum, apoptosis was measured by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling （TUNEL） assay. The expression levels of cleaved caspase-3 and cleaved poly（ADP-ribose） polymerase （PARP） were detected by Western blot.RESULTS： The recombinant Foxo3a expression plasmid was constructed successfully. The apoptotic rates of SGC7901 cells transfected with 1 and 2μg of Foxo3a expression plasmid were 5.8% ±2.3% and 11.1% ±3.4%, respectively, significantly higher than those of control cells. The activity of caspase-3 and PARP was significantly higher in transfected cells than in control cells.CONCLUSION： Foxo3a can promote apoptosis and inhibit proliferation in SGC7901 cells perhaps via upregulation of caspase-3 and PARP activity.

关 键 词：FOXO3A 胃癌细胞 细胞凋亡 信号通路 

分 类 号：R735.2[医药卫生—肿瘤] R730.23[医药卫生—临床医学]