RASSF1A基因启动子区甲基化在胃癌组织中的检测  

Detection of RASSF1A promoter methylation in gastric cancer

作　　者：刘培[1] 姜相君[1] 葛银林[2] 

机构地区：[1]青岛大学医学院附属青岛市市立医院消化内科,山东省青岛市266011 [2]青岛大学医学院生物化学与分子生物学教研室,山东省青岛市266021

出　　处：《世界华人消化杂志》2009年第30期3144-3147,共4页World Chinese Journal of Digestology

摘　　要：目的:检测胃癌组织中RASSF1A基因启动子区甲基化状况,并探讨其与临床病理特征的关系.方法:采用甲基化特异性聚合酶链反应(methylation-specific PCR,MSP)检测39例胃癌组织,及相应癌旁组织和30例对照组织中RASSF1A基因启动子区甲基化水平.结果:胃癌组织中RASSF1A基因启动子区甲基化率显著高于癌旁组织甲基化率及对照组(64.1%vs7.7%,0%,均P<0.01).胃癌组织中不同年龄、性别、分化程度及淋巴结转移与否的RASSF1A基因甲基化率的差异均无统计学意义.结论:RASSF1A基因启动子区甲基化与胃癌的发生密切相关,MSP法是一种快速、敏感的基因甲基化检测方法,可用于胃癌的辅助诊断.AIM： To detect RAS association domain family protein 1A （RASSF1A） promoter methylation and analyze its correlation with clinical and pathological parameters in gastric cancer. METHODS： Methylation-specific polymerase chain reaction （MSP） was used to detect RASSF1A promoter methylation in tumor and tumoradjacent tissues from 39 gastric cancer patients and in 30 control specimens from superficial gastritis patients or healthy volunteers. RESULTS： The rate of RASSF1A promoter methylation was significantly higher in gastric cancer than in tumor-adjacent tissues and control specimens （64.1% vs 7.7% and 0%, respectively; both P〈0.01）. No correlation was found between RASSF1A promoter methylation andage, gender, tumor differentiation or lymph node metastasis in patients with gastric cancer. CONCLUSION： RASSF1A promoter methylation is closely associated with the oncogenesis of gastric cancer. MSP is a novel and specific method for detection of promoter region methylation and can be used to diagnose gastric carcinoma.

关 键 词：胃癌 RASSF1A基因 甲基化 甲基化特异性聚合酶链反应 

分 类 号：R735.2[医药卫生—肿瘤] R734.2[医药卫生—临床医学]