肠易激综合征患者肠黏膜SP、SPR和5-HT的变化  

Changes in substance P, substance P receptor and 5-hydroxytryptamine expression in the ileocecal mucosa in patients with irritable bowel syndrome

作　　者：夏頔[1] 刘希双[1] 鞠辉[1] 

机构地区：[1]青岛大学医学院附属医院内镜诊治科,山东省青岛市266003

出　　处：《世界华人消化杂志》2009年第30期3169-3173,共5页World Chinese Journal of Digestology

摘　　要：目的:观察肠易激综合征(IBS)患者回盲部肠黏膜P物质(SP)、P物质受体(SPR)及5-羟色胺(5-HT)的改变及其相互关系,探讨其在IBS病理、生理机制中可能的作用与临床意义.方法:采用EnVision免疫组织化学染色法对IBD患者回盲部黏膜组织切片的SP与SPR进行染色,5-HT用SP免疫组织化学染色法,用彩色病理图像分析软件及免疫组织化学分析软件进行分析.结果:与对照组比较IBS组SP的阳性强度均值、阳性面积均显著增大,差别有统计学意义(F=6.463,P<0.05),其中IBS-D和IBS-C与对照组比较差别有高度统计学意义(Q=4.231、3.463,均P<0.01);IBS组和对照组相比,回盲部黏膜SPR、5-HT阳性细胞明显增多(均P<0.05),且SPR和5-HT密切相关(r=0.1579,P<0.05).结论:SP、SPR及5-HT相互作用在IBS内脏高敏感的病理生理过程中可能具有重要的作用.AIM： To investigate the changes in substance P （SP）, substance P receptor （SPR） and 5-hydroxytryptamine （5-HT） expression in the ileocecal mucosa in patients with irritable bowel syndrome （IBS） and elucidate their possible roles in the development of IBS. METHODS： The expression of SP and SPR in the ileocecal mucosa was detected by immunohistochemistry using the Envision system. The expression of 5-HT was detected by immunohistochemistry using the streptavidin-peroxidase method. The staining results were analyzed qualitatively and quantitatively using a color image analyzer. RESULTS： The positive intensity and area of SP staining in the ileocecal mucosa were signifi-cantly higher in IBS patients than in normal controls （F = 6.463, P〈0.05）. Especially, the positive intensity and area of SP staining in the ileocecal mucosa were greater in IBS-D and IBS-C patients than in control individuals （Q = 4.231 and 3.463, respectively; both P〈0.01）. The number of SPR and 5-HT-positive cells in the ileocecal mucosa were significantly higher in patients with IBS than in control individuals （P〈0.05）. SPR expression was closely correlated with 5-HT expression in the ileocecal mucosa of IBS patients （r = 0.1579, P〈0.05）. CONCLUSION： 5-HT, SP and SPR may play a central role in the gut hypersensitivity in both motor response and visceral perception in IBS.

关 键 词：肠易激综合征 P物质 P物质受体 5-羟 色胺 

分 类 号：R574[医药卫生—消化系统]