骨桥蛋白与缺氧诱导因子-1在大肠腺瘤及癌组织中的表达及意义  

Significance of osteopontin and hypoxia-inducible factor-α expression in colorectal carcinoma and adenoma

作　　者：袁禧先[1] 马晓颖[2] 王凤荣[2] 王喆[3] 王晓[4] 王树鸿[2] 

机构地区：[1]佳木斯大学附属第一医院消化二科,黑龙江省佳木斯市154003 [2]佳木斯大学临床医学院,黑龙江省佳木斯市154003 [3]日本金泽大学大学院医学系研究科 [4]佳木斯大学附属第一医院病理科,黑龙江省佳木斯市154003

出　　处：《世界华人消化杂志》2009年第30期3174-3179,共6页World Chinese Journal of Digestology

摘　　要：目的:探讨骨桥蛋白(OPN)及缺氧诱导因子-1α(HIF-1α)在大肠癌、大肠腺瘤及大肠正常组织中的表达程度及其与临床病理学之间的关系.方法:采用免疫组织化学SABC法检测50例大肠癌及24例大肠腺瘤、12例正常大肠组织病理标本中OPN及HIF-1α的表达.结果:OPN及HIF-1α在正常组织中无表达,在大肠癌组织、腺瘤组织中为高表达.OPN的表达率在腺瘤组与肠癌组间比较差别无显著性.HIF-1α的表达率在腺瘤组与肠癌组间比较,差别有显著性(33.33%vs62.00%,P<0.05).二者在肠癌组中的表达与肿瘤的浸润深度、淋巴结转移、Dukes分期呈正相关;与患者的性别、年龄、肿瘤的大小则无相关性.OPN与HIF-1α在大肠癌组织中的表达具有明显的相关性(r=0.700,P<0.01).结论:OPN及HIF-1α在大肠癌的浸润和转移中起重要作用;HIF-1α高表达的肿瘤组织具有较强的浸润与转移能力.AIM： To investigate the expression of osteopontin （OPN） and hypoxia-inducible factor-α （HIF-1α） and analyze their correlation with clinical and pathophysiological parameters in colorectal carcinoma and adenoma. METHODS： The expression of OPN and HIF-1α in 50 colorectal carcinoma specimens, 24 colorec-tal adenoma specimens and 12 normal colorectal tissues were examined by immunohistochemistry using the streptavidin-peroxidase method. RESULTS： OPN and HIF-1α expression was not detected in normal colorectal tissues. In contrast, OPN and HIF-1α were highly expressed in colorectal carcinoma and adenoma. Although no significant difference was noted in OPN expression level between colorectal adenoma and carcinoma, significant difference was found in HIF1α expression level between colorectal adenoma and carcinoma （33.33% vs 62.00%, P〈0.05）. Both OPN and HIF-1α expression were positively correlated with lymph node metastasis, depth of tumor invasion and Dukes’ stage, but not with patients＇ gender and age. A significant correlation was noted between the expression of OPN and HIF-1α in colorectal carcinoma （r = 0.700, P〈0.01）. CONCLUSION： OPN and HIF-1α may play an important role in the invasion and metastasis of colorectal carcinoma. Tumors expressing high level of HIF-1α have greater invasive and metastatic capacity.

关 键 词：骨桥蛋白 缺氧诱导因子-1Α 大肠腺瘤 大肠癌 免疫组织化学 

分 类 号：R735.34[医药卫生—肿瘤] R543.5[医药卫生—临床医学]