TACE-TGF-α相互作用参与胚泡着床的机制研究  

The regulation investigation of the interaction between TACE and TGF-α

作　　者：杨环[1] 朱兴春[2] 杨戎[1] 张文文[1] 刘帅[1] 

机构地区：[1]重庆医科大学公共卫生学院生殖生理教研室,重庆400016 [2]川北医学院检验科,南充637000

出　　处：《重庆医科大学学报》2009年第11期1466-1469,共4页Journal of Chongqing Medical University

基　　金：重庆市科委自然科学基金项目(CSTC;2007BB5273)

摘　　要：目的:探讨胚泡着床过程中转化生长因子α(Transforming growth factor-α,TGF-α)对肿瘤坏死因子-α转化酶(Tumor necrosis factor-converting enzyme,TACE/ADAM17)的反馈调控机制。方法:用逆转录聚合酶链反应(Reverse transcription polymerase chain reaction,RT-PCR)和免疫组化的方法分别检测实验组(注射TGF-α抗体)和对照组(注射小鼠血清)后子宫内膜TACE mRNA和蛋白的表达。结果:对照组TACE mRNA和蛋白明显高于实验组。结论:在胚胎着床过程中,TGF-α能反馈调控TACE在子宫内膜的表达,TACE-TGF-α-EGFR途径可能是胚胎着床的调控机制之一。Objective：To investigate the feedback regulation of transforming growth factor α （TGF-α） to tumor necrosis factor-α converting enzyme. Methods： Reverse transcription polymerase chain reaction （RT-PCR） and immunochemistry were applied to detect TACE mRNA and protein in mice endometria of experiment group （TGF-α antibody was injected） and control group （saline was injected）. Results ： The expressions of TACE mRNA and protein in control group were higher than in experiment group. Conclusion： TGF-α could feedback on TACE expression in mice endometria and TACE-TGF-α-EGFR might one of the regulations during blastocyst implantation.

关 键 词：胚胎着床 肿瘤坏死因子-α转化酶 转化生长因子Α 子宫内膜 免疫组化 逆转录聚合酶链反应 

分 类 号：R394[医药卫生—医学遗传学]