浸润性T淋巴细胞及Th细胞因子在不同类型乳腺癌中的表达  

Expression of infiltrating T lymphocytes and T helper cytokines in different types of breast cancer

作　　者：徐新生[1] 郭晓静[1] 刘芳芳[1] 李伟东[1] 刘冰冰[1] 谷峰[1] 郎荣刚[1] 范宇[1] 付丽[1] 

机构地区：[1]天津医科大学附属肿瘤医院乳腺病理科教育部乳腺癌研究重点实验室,天津300060

出　　处：《中华乳腺病杂志（电子版）》2009年第6期28-33,共6页Chinese Journal of Breast Disease(Electronic Edition)

基　　金：国家自然科学基金资助项目(30600225);教育部长江学者奖励计划乳腺癌创新团队(IRT0743);国家"863"计划项目(2006AA02A249);国家"973"计划项目(2009CB521700)

摘　　要：目的研究浸润性T淋巴细胞和Th细胞因子在不同类型乳腺癌中表达的意义。方法利用免疫组织化学链霉亲和素生物素法(labelled streptavidin-biotin method,LSAB)方法检测61例浸润性微乳头状癌(invasive micropapillary carcinoma,IMPC)、71例浸润性导管癌(invasive ductal carcinoma,IDC)和27例髓样癌(medullary carcinoma,MC)3种乳腺癌中浸润T淋巴细胞亚群分布以及Th细胞因子的表达。T淋巴细胞组间比较采用Kruskal-WallisTest,组内比较采用Wilcoxon Signed RanksTest;组间Th细胞因子的比较采用χ2检验。结果CD3、CD8和CD4均表达于淋巴细胞胞膜。CD3+T细胞在3组间差异无统计学意义(P=0.735)。CD8+T和CD4+T细胞在3组间差异均有统计学意义(P=0.000,P=0.012)。Th细胞因子主要表达于肿瘤细胞和淋巴细胞的胞质。IL-2表达在IMPC组最低(25/61,41.0%),其次是IDC组(33/71,46.5%),最高是MC组(20/27,74.1%),3组间差异有统计学意义(P=0.014);IFN-γ的表达类似于IL-2,IMPC组最低(39/61,63.9%),其次是IDC组(57/71,80.3%),最高是MC组(24/27,88.9%),3组间差异有统计学意义(P=0.019);IL-4的表达在IDC组最高(42/71,59.2%),其次是IMPC组(19/61,31.1%),最低是MC组(5/27,18.5%),3组间差异有统计学意义(P=0.000);IL-10的阳性率在IMPC组是87.8%(47/61),在IDC组是87.3%(62/71),在MC组是88.9%(24/27),3组间差异无统计学意义(P>0.050)。结论浸润性T淋巴细胞和Th细胞因子在不同类型乳腺癌中表达不同。Objective To study the expression of tumor-infiltrating lymphocytes （TILs） and T helper cytokines in invasive micropapillary carcinoma （IMPC）, invasive ductal carcinoma （IDC） and medullary carcinoma （MC） of the breast. Methods Three T-cell markers including pan-T cell marker （CD3）, T-helper cell marker （CD4）, cytotoxie T cell marker （CD8）, and four T-helper cytokines including interleukin-2 （IL-2）, interferongamma （IFN-γ）, interleukin-4 （IL-4）, and interleukin-10 （IL-10） were tested immunohistochemically in the three groups, （IMPC n=61, IDC n=71, and MC n=27） on formalin-fixed, paraffin embedded tumors. Kruskal-Wallis Test and Wilcoxon Signed Ranks Test were used for analysis of TILs;chi-square test was used for analysis of T helper cytokines. Results Markers, CD3, CD4 and CDS, all expressed in the cell membrane of lymphocytes. There was no statistical difference in CD3＋T cells number between the three groups （P= 0. 705）; CD8^＋ T cells and CD4^＋ T cells were statistically different between the three groups （P=0. 000,P=0. 012）. T helper cytokines expressed in cytoplasm of tumour cells and lymphocytes. The expression of IL-2 was the highest in the MC group （20/27,74. 1%）, followed by the IDC group （33/71,46. 5%）, and the lowest in the IMPC group （25/61, 41.0%）, there was a statistical difference between the three groups（P =0. 014）. The expression of IFN-γ was the highest in the MC group （24/27,88. 9%） followed by the IDC group（57/71,80. 3%）, and the lowest in the IMPC group（39/61,63.9%）, there was a statistical difference between the three groups（P = 0. 019）. The positive expression of IL-4 was the highest in the IDC group （42/71,59. 2%）, followed by the IMPC group （19/61, 31.1%） and the lowest in the MC group （5/27, 18. 5%）, there was a statistical difference between the three groups （P=0. 000）. The positive rate of IL-10 expression was 87.8%（47/61）in the IMPC group, 87.3%（62/71） in the IDC group

关 键 词：乳腺肿瘤 浸润T淋巴细胞 TH细胞因子 

分 类 号：R737.9[医药卫生—肿瘤]