RECK基因重组慢病毒载体的构建与鉴定  

Construction and Identification of RECK Gene Recombinant Lentiviral Vector

作　　者：周进[1] 李德春[1] 田青水[2] 

机构地区：[1]苏州大学附属第一医院普外科,江苏苏州215006 [2]连云港市第一人民医院普外科,江苏连云港222000

出　　处：《中国现代医生》2009年第35期13-14,共2页China Modern Doctor

摘　　要：目的构建RECK基因慢病毒载体,为体内外实验研究提供依据。方法PCR技术构建慢病毒载体表达质粒pGC-FU-RECK,PCR鉴定、测序验证RECK基因,并将其和包装质粒pHelper1.0、pHelper2.0共转染293T细胞,包装产生重组慢病毒GC-FU-RECK。结果pGC-FU-RECK中携有正确的RECK基因,并能在293T细胞中表达;能产生重组病毒GC-FU-RECK。结论成功构建了RECK基因的重组慢病毒载体,为研究其在胰腺癌中的生物学功能奠定基础。Objective To construct a lentiviral vector carrying RECK gene and provide the basis for further experiments. Methods The lentiviral expression vector, pGC-FU-RECK was identfied by PCR technique. Recombinant lentiviruses were produced by 293T cells following the co-transfection with packaging plasmids-pHelper 1.0 and pHelper 2.0. Results Plasmid pGC-FU-RECK carried the correct RECK gene and could be expressed in 293T cells, and could be produced by co-transfection of pGC-FU-RECK. Conclusion The recombinant lentivirus GC-FU-RECK can be produced successfully,which will provide the basis for the further studies on the function of RECK gene in pancreatic adenocarcinoma.

关 键 词：RECK 慢病毒 

分 类 号：R782[医药卫生—口腔医学]