四种梅毒血清检验的方法学比较  

Methodology Comparison of Four Serological Test Methods for Syphilis

作　　者：黄雪梅[1] 蒲泽晏 刘方久[1] 

机构地区：[1]遂宁市中心医院,四川遂宁629000

出　　处：《四川生殖卫生学院学报》2009年第6期63-64,共2页Journal of Sichuan Reproductive Health Institute

摘　　要：目的：由于我室现在输血前检查以及梅毒的检测量越来越大，特对四种梅毒检测方法进行对比分析，建立适合我室梅毒的检测程序。方法：用ELISA法检测我院去年10月28日到今年6月的输血前检查标本以及门诊的要求查梅毒的标本10255例，其中阳性者再用RPR法．TRUST法和TPPA进行检测。结果：ELISA共检出阳性295例，其中ELISA阳性，TPPA为阳性的有290例，符合率为98．3％。ELISA的假阳性率为1．7％。295例TPPA阳性标本中RPR和TRUST法检出阳性为102例，检出率为34．58％，RPR和TRUST法漏检率为65．42％。而且RPR和TRUST法中102例阳性在做TPPA确证实验时滴度大于或等于1：5120的有72例，193例阴性在做TPPA确证实验时滴度大于或等于1：5120的仅有18例。结论：ELISA可用于我室输血前检查的筛查实验，TPPA可作为检测ELISA中阳性的确证实验。TPPA确证实验梅毒滴度越高，RPR法和TRUST法检出阳性的可能性就越大。Objective： To make comparative analysis on four kinds of serological test for syphilis to establish suitable detection program for our laboratory. Methods： Treponema pallidum antibodies were measured with ELISA of 10255 pretransfusion patient and outpatient serums, positive samples detected with RPR, TRUST and TPPA methods. Results： 295 positive cases were detected with ELISA, 290 positive cases were detected with ELISA and TPPA, the positive coincidence rate of ELISA and TPPA was 98.3%. 102 positive cases were detected with RPR and TRUST in 295 TPPA positive cases. The detecting rate was 34.58%, and the missing rate of RPR and TRUST was 65.42%. 72 cases were detected in 102 positive cases with TPPA confirmatory test （the titers of Treponema pallidum antibody reached to 1： 5120）, only 18 cases were detected in 193 negative cases with TPPA confirmatory test. Conclusion： ELISA can be used to pretransfusion screening test and TPPA can be used to confirmatory test. The higher the titers of Treponema pallidum antibody with TPPA confirmatory test, the bigger possibility of the positive with RPR and TRUST positive.

关 键 词：ELISA RPR TPPA TRUST 

分 类 号：R44[医药卫生—诊断学]