青蒿总香豆素抗热应激作用及其机理的研究  

Studies on Anti-heat-stress Activity and Its Mechanisms of Total Coumarins from Artemisia annua

作　　者：闫寒[1] 李爱媛[2] 赵一[2] 林启云[2] 李沧海[1] 

机构地区：[1]中国中医科学院医学实验中心,北京100700 [2]广西中医学院,广西南宁530001

出　　处：《中国实验方剂学杂志》2009年第12期98-100,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重点基础研究发展计划项目(2006CB504701);北京市自然科学基金(7073094)

摘　　要：目的:研究青蒿抗热应激作用及其机理。方法:利用高温高湿环境造成家兔热应激模型,观察青蒿总香豆素对家兔肛温上升及血液、肺组织磷脂酶A2活性和循环内皮细胞数目的变化,并测定其对脑、心脏、肾组织钠泵活性及小鼠抗脑缺氧能力的影响。结果:青蒿总香豆素可明显降低热应激家兔体温上升速度,并伴随血清、肺组织磷脂酶A2活性降低,循环内皮细胞减少,显著抑制脑、心、肾组织钠泵活性,延长断头小鼠张口喘气时间几近1倍。结论:香豆素部位可能是青蒿祛暑功效的活性成分群之一。Objective：To study the Anti-heat-stress activity and related mechanisms of total coumarins（TCs）from Artemisia annua.Methods： Heat stress rabbit model caused by high temperature and high humidity was used and rectal temperature（Tr） responses,PLA2 activity in blood and lung,circulating endothelial cells（CECs） were measured,moreover,Na^＋,K^＋-ATPase activity in brain,kedney and heart in rabbits was assayed,gasping time after decapitation in mice was observed after treatment of total coumarins（TCs） from Artemisia annua.Results： Administration of TCs reduced the rise of rectal temperature and CECs in heat stress rabbits,inhibited the activity of the PLA2 and Na^＋,K^＋-ATPase,increased the gasping time in mice.Conclusion： Coumarins are one of anti-heat-stress active fractions in Artemisia annua.

关 键 词：黄花蒿 香豆素 热应激 磷脂酶A2 钠泵 循环内皮细胞 

分 类 号：R285.5[医药卫生—中药学]