戊四氮致痫后大鼠海马中Caspase-3和Hsp70的动态表达变化  

The expession changes of caspase-3 and hsp70 in hippocamp of rats after PTZ-induced seizure

作　　者：甄军丽[1] 王维平[1] 安立伟[1] 李周平[1] 贾丽景[1] 

机构地区：[1]河北医科大学第二医院神经内科,河北石家庄050000

出　　处：《中风与神经疾病杂志》2009年第6期664-667,共4页Journal of Apoplexy and Nervous Diseases

基　　金：河北省科技攻关项目(07276101D-21);河北省自然科学基金资助项目(C2007000852)

摘　　要：目的研究戊四氮(pentlenetrazol,PTZ)致癫痫发作后大鼠海马中caspase-3和hsp70的动态变化,以便及时进行干预并控制凋亡的发生。方法PTZ致痫急性发作后分别在3、6、12、24h,行免疫组化法计算阳性细胞的平均光密度值;Nissle染色法检测神经细胞丢失坏死情况;电镜观察海马CA3区的超微结构,Western blot方法分别检测海马组织中caspase-3和hsp70的表达情况。结果致痫后caspase-3和hsp70的表达趋势在各种方法中基本一致,即3h开始表达增高,6h后明显增高,24h时神经细胞表现典型的凋亡状态,明显高于对照组(P<0.05)。结论癫痫发作同时上调了caspase-3和hsp70的表达,可导致神经细胞发生凋亡,从而表明癫痫急性期诱导的细胞凋亡可能对脑组织在应激保护方面起了重要的角色。Objective To investigate the dynamic state changes of caspases-3 and hsp70 in hippocamp of rats after seizure so that we can adopt intervention and control neurocyte apoptosis in time.Methods At 3h,6h,12h and 24h after seizure,the average optical density of positive cell was counted with immunohistochemistry.The situation of neurocyte loss or necrosis was determined with nissle staining and ultramicrostructure of hippocamp CA3 was observed with electron microscope method,respectively.Expressions of caspase-3 and hsp70 in hippocamp of rats were determined by means of Western blot.Results There was a similar tendency between caspase-3 and hsp70 in each method.There was significantly higher than that control group（P〈0.05）.Namely,they began to increase slightly at 3h after seizure and at 6h after seizure significantly,then the neurocytes showed a typical apoptosis state at 24h seizure.Conclusion Seizure could upregulate expression of caspase-3 and hsp70 simultaneously,and lead to neurocyte apoptosis.This result demonstrates that neurocyte apoptosis at acute stage of seizure might play an important role in stress defendence of brian tissue.

关 键 词：癫痫发作 凋亡 CASPASE-3 HSP70 海马 

分 类 号：R742.1[医药卫生—神经病学与精神病学]