液相色谱-串联质谱法对乳制品中吡喹酮残留量的测定  

Determination of praziquantel residues in milk products by liquid chromatography-tandem mass spectrometry

作　　者：欧阳少伦[1] 林峰[1] 吴映璇[1] 邵琳智[1] 姚仰勋[1] 

机构地区：[1]广东出入境检验检疫局检验检疫技术中心,广州510623

出　　处：《中国卫生检验杂志》2009年第12期2745-2746,2749,共3页Chinese Journal of Health Laboratory Technology

基　　金：国家认证认可监督管理委员会资助课题(20082267-T-469)

摘　　要：目的：建立液相色谱-串联质谱法测定乳制品中吡喹酮残留的方法。方法：样品经乙腈-二氯甲烷或乙酸乙酯提取,经吹氮气浓缩,正己烷脱脂。结果：方法经过精密度和回收率验证,测定低限为5.0μg/kg。添加水平为5.0～50.0μg/kg时,样品回收率为82%～108%,相对标准偏差为2.6%～12.7%。结论：该方法样品前处理简单、灵敏度高、重现性好,适用于乳制品中吡喹酮检测。Objective：To develop a LC-MS/MS method for determination of Praziquantel in milk products.Methods：Sample are extracted with acetonitrile-dichloromethane or ethylacetate,Concentrates with the nitrogen,N-hexane defatted.The method in the present study was well verified and good results were obtained with respect to precision and spiked recovery.Results：The limit of quntitation was 5.0 μg/kg.When the spiking level was 5.0～50.0 μg/kg,the average recoveries were 82%～108%.The relative standard deviations（RSD） was 2.6%～12.7%.Conclusion：The method is easy to operate and of high sensitivity and good repeatability for determination of praziquantel residues in milk products.

关 键 词：吡喹酮 液相色谱-串联质谱 乳制品 

分 类 号：O657.72[理学—分析化学]